Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/13087
ชื่อเรื่อง: Multiplex Paper-Based Colorimetric DNA Sensor Using Pyrrolidinyl Peptide Nucleic Acid-Induced AgNPs Aggregation for Detecting MERS-CoV, MTB, and HPV Oligonucleotides
ผู้แต่ง: Teengam P.
Siangproh W.
Tuantranont A.
Vilaivan T.
Chailapakul O.
Henry C.S.
Keywords: Amino acids
Analytic equipment
Assays
Color
Colorimetry
DNA
Ionic strength
Nanoparticles
Oligonucleotides
Paper
Peptides
Probes
RNA
Silver
Complementary detection
DNA and RNA detection
Electrostatic repulsion
Mycobacterium tuberculosis
Nanoparticle aggregation
Paper-based analytical devices
Silver nanoparticles (AgNps)
Single-base mismatch
Nucleic acids
Concentration
Detection
Nucleic Acids
Screening
bacterial DNA
DNA
metal nanoparticle
oligonucleotide
peptide nucleic acid
silver
virus DNA
chemistry
colorimetry
genetics
human
image processing
limit of detection
metabolism
Middle East respiratory syndrome coronavirus
Mycobacterium tuberculosis
nucleic acid hybridization
nucleotide sequence
paper
Papillomaviridae
procedures
Base Sequence
Colorimetry
DNA
DNA, Bacterial
DNA, Viral
Humans
Image Processing, Computer-Assisted
Limit of Detection
Metal Nanoparticles
Middle East Respiratory Syndrome Coronavirus
Mycobacterium tuberculosis
Nucleic Acid Hybridization
Oligonucleotides
Paper
Papillomaviridae
Peptide Nucleic Acids
Silver
วันที่เผยแพร่: 2017
บทคัดย่อ: The development of simple fluorescent and colorimetric assays that enable point-of-care DNA and RNA detection has been a topic of significant research because of the utility of such assays in resource limited settings. The most common motifs utilize hybridization to a complementary detection strand coupled with a sensitive reporter molecule. Here, a paper-based colorimetric assay for DNA detection based on pyrrolidinyl peptide nucleic acid (acpcPNA)-induced nanoparticle aggregation is reported as an alternative to traditional colorimetric approaches. PNA probes are an attractive alternative to DNA and RNA probes because they are chemically and biologically stable, easily synthesized, and hybridize efficiently with the complementary DNA strands. The acpcPNA probe contains a single positive charge from the lysine at C-terminus and causes aggregation of citrate anion-stabilized silver nanoparticles (AgNPs) in the absence of complementary DNA. In the presence of target DNA, formation of the anionic DNA-acpcPNA duplex results in dispersion of the AgNPs as a result of electrostatic repulsion, giving rise to a detectable color change. Factors affecting the sensitivity and selectivity of this assay were investigated, including ionic strength, AgNP concentration, PNA concentration, and DNA strand mismatches. The method was used for screening of synthetic Middle East respiratory syndrome coronavirus (MERS-CoV), Mycobacterium tuberculosis (MTB), and human papillomavirus (HPV) DNA based on a colorimetric paper-based analytical device developed using the aforementioned principle. The oligonucleotide targets were detected by measuring the color change of AgNPs, giving detection limits of 1.53 (MERS-CoV), 1.27 (MTB), and 1.03 nM (HPV). The acpcPNA probe exhibited high selectivity for the complementary oligonucleotides over single-base-mismatch, two-base-mismatch, and noncomplementary DNA targets. The proposed paper-based colorimetric DNA sensor has potential to be an alternative approach for simple, rapid, sensitive, and selective DNA detection. © 2017 American Chemical Society.
URI: https://ir.swu.ac.th/jspui/handle/123456789/13087
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85020652243&doi=10.1021%2facs.analchem.7b00255&partnerID=40&md5=74a933111e5dadb18c00a1e6dc1b8214
ISSN: 32700
Appears in Collections:Scopus 1983-2021

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