Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/13044
ชื่อเรื่อง: Sensory acceptable equivalent doses of β-phenylethyl isothiocyanate (PEITC) induce cell cycle arrest and retard the growth of p53 mutated oral cancer in vitro and in vivo
ผู้แต่ง: Lam-Ubol A.
Fitzgerald A.L.
Ritdej A.
Phonyiam T.
Zhang H.
Myers J.N.
Huang P.
Trachootham D.
Keywords: Cytology
Diseases
Tumors
Cell-cycle arrest
Cruciferous vegetables
Equivalent dose
Functional ingredient
Isothiocyanates
Nuclear translocations
Oral cancer cells
Tumor progressions
Cells
antineoplastic agent
isothiocyanic acid derivative
phenethyl isothiocyanate
protein p53
animal
apoptosis
cell cycle checkpoint
cell proliferation
drug effect
genetics
human
male
metabolism
mouse
mouth tumor
nude mouse
pathophysiology
taste
tumor cell line
Animals
Anticarcinogenic Agents
Apoptosis
Cell Cycle Checkpoints
Cell Line, Tumor
Cell Proliferation
Humans
Isothiocyanates
Male
Mice
Mice, Nude
Mouth Neoplasms
Taste
Tumor Suppressor Protein p53
วันที่เผยแพร่: 2018
บทคัดย่อ: High doses of β-phenylethyl isothiocyanate (PEITC), a phytochemical in cruciferous vegetables, are not feasible for consumption due to a strong mouth-tingling effect. This study investigated the anti-cancer effect of PEITC at sensory acceptable doses. In vitro, PEITC was selectively toxic to oral cancer cells (CAL-27, FaDu, SCC4, SCC 9, SCC15, SCC25 and TU138), compared to oral keratinocytes (OKF6/TERT2 and NOK/Si). In vivo, 5 and 10 mg kg-1 PEITC, equivalent to human organoleptically acceptable doses, retarded tumor growth and prolonged the survival of mice bearing p53-mutated oral cancer cells-TU138 xenograft. Mechanistically, PEITC induced ROS accumulation, nuclear translocation of p53 and p21 and G1/S cell cycle arrest in vitro; increased p53 and 8-oxo-dG levels; and decreased Ki-67 intense/mild staining ratios without TUNEL changes in vivo. These findings suggested that the sensory acceptable doses of PEITC selectively induced ROS-mediated cell cycle arrest leading to delayed tumor progression and extended survival. PEITC could be a functional ingredient for oral cancer prevention. © The Royal Society of Chemistry.
URI: https://ir.swu.ac.th/jspui/handle/123456789/13044
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85050534112&doi=10.1039%2fc8fo00865e&partnerID=40&md5=4c35f2d423e2df95961aa58187950a97
ISSN: 20426496
Appears in Collections:Scopus 1983-2021

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