Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/13039
ชื่อเรื่อง: Porphyromonas gingivalis elevated high-mobility group box 1 levels after myocardial infarction in mice
ผู้แต่ง: Srisuwantha R.
Shiheido Y.
Aoyama N.
Sato H.
Kure K.
Laosrisin N.
Izumi Y.
Suzuki J.-I.
Keywords: high mobility group B1 protein
biological marker
high mobility group B1 protein
HMGB1 protein, mouse
animal cell
animal model
animal tissue
Article
bacterial growth
bacterial infection
controlled study
enzyme linked immunosorbent assay
heart infarction
histopathology
immunohistochemistry
male
mouse
nonhuman
periodontal disease
Porphyromonas gingivalis
priority journal
protein expression
animal
Bacteroidaceae infection
biosynthesis
C57BL mouse
cardiac muscle
cardiac muscle cell
complication
disease model
isolation and purification
metabolism
microbiology
Myocardial Infarction
pathology
Porphyromonas gingivalis
Animals
Bacteroidaceae Infections
Biomarkers
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
HMGB1 Protein
Immunohistochemistry
Male
Mice
Mice, Inbred C57BL
Myocardial Infarction
Myocardium
Myocytes, Cardiac
Porphyromonas gingivalis
วันที่เผยแพร่: 2017
บทคัดย่อ: High mobility group box 1 (HMGB1) is a nuclear protein released from necrotic cells, inducing inflammatory responses. Epidemiological studies suggested a possible association between periodontitis and cardiovascular diseases (CVDs). Due to tissue damage and necrosis of cardiac cells following myocardial infarction (MI), HMGB1 is released, activating an inflammatory reaction. However, it remains unclear whether periodontitis is also involved in myocardial damage. The purpose of this study was to determine the effect of the periodontal pathogen Porphyromonas gingivalis (P.g.) after MI in mice. C57BL/6J wild type mice in post-MI were inoculated with P.g. in the infected group (P.g.-inoculated MI group) and with phosphate buffer saline (PBS) in the control group (PBS-injected MI group). Plasma samples and twelve tissue samples from mice hearts after MI were obtained. We determined the expression of HMGB1 by ELISA and immunohistochemistry. The level of HMGB1 protein in the P.g.-inoculated MI group was significantly higher than in the PBS-injected MI group on day 5, but not on day 14. Immunohistochemistry analysis revealed that HMGB1 was mainly expressed in cardiomyocytes, immune cells, and vascular endothelial cells in the PBS-injected MI group, while HMGB1 was seen broadly in degenerated cardiomyocytes, extracellular fields, immune cells, and vascular endothelial cells in the P.g.-inoculated MI group. A significant increase in the number of HMGB1 positive cells was observed in the P.g.-inoculated MI group compared to the PBS-injected MI group. Infection with P.g. after MI enhanced myocardial HMGB1 expression. There is a possible relationship between periodontitis and post-infarction myocardial inflammation through HMGB-1. © 2017, International Heart Journal Association. All rights reserved.
URI: https://ir.swu.ac.th/jspui/handle/123456789/13039
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85032175240&doi=10.1536%2fihj.16-500&partnerID=40&md5=0f073f5ffb20054b70162c93615a6866
ISSN: 13492365
Appears in Collections:Scopus 1983-2021

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