Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/12996
ชื่อเรื่อง: Poly(DL-lactide)-degrading enzyme production by immobilized Actinomadura keratinilytica strain T16-1 in a 5-L fermenter under various fermentation processes
ผู้แต่ง: Panyachanakul T.
Kitpreechavanich V.
Tokuyama S.
Krajangsang S.
Keywords: Bacteria
Biodegradable polymers
Biodegradation
Bioreactors
Cell immobilization
Enzyme activity
Enzymes
Fermentation
Fermenters
Lactic acid
Process control
Productivity
Radioactive waste vitrification
Actinomycete
Batch fermentation
Continuous fermentation
Depolymerase
Fermentation process
Optimized conditions
Plastic wastes
Poly(DL-lactic acid)
Enzyme immobilization
polylactide
proteinase
Actinomadura
Actinomadura keratinilytica
aeration
Article
biotechnological production
continuous fermentation
enzyme activity
enzyme synthesis
fermentation
fermentation optimization
immobilized cell
nonhuman
repeated batch fermentation
scale up
วันที่เผยแพร่: 2017
บทคัดย่อ: Background Poly(DL-lactic acid), or PDLLA, is a biodegradable polymer that can be hydrolyzed by various types of enzymes. The protease produced by Actinomadura keratinilytica strain T16-1 was previously reported to have PDLLA depolymerase activity. However, few studies have reported on PDLLA-degrading enzyme production by bacteria. Therefore, the aims of this study were to determine a suitable immobilization material for PDLLA-degrading enzyme production and optimize PDLLA-degrading enzyme production by using immobilized A. keratinilytica strain T16-1 under various fermentation process conditions in a stirrer fermenter. Results Among the tested immobilization materials, a scrub pad was the best immobilizer, giving an enzyme activity of 30.03 U/mL in a shake-flask scale. The maximum enzyme activity was obtained at aeration 0.25 vvm, agitation 170 rpm, 45°C, and 48 h of cultivation time. Under these conditions, a PDLLA-degrading enzyme production of 766.33 U/mL with 15.97 U/mL·h productivity was observed using batch fermentation in a 5-L stirrer fermenter. Increased enzyme activity and productivity were observed in repeated-batch (942.67 U/mL and 19.64 U/mL·h) and continuous fermentation (796.43 U/mL and 16.58 U/mL·h) at a dilution rate of 0.013/h. Scaled-up production of the enzyme in a 10-L stirrer bioreactor using the optimized conditions showed a maximum enzyme activity of 578.67 U/mL and a productivity of 12.06 U/mL·h. Conclusions This research successfully scaled-up the enzyme production to 5 and 10 L in a stirrer fermenter and is helpful for many applications of poly(lactic acid). © 2017
URI: https://ir.swu.ac.th/jspui/handle/123456789/12996
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85034060952&doi=10.1016%2fj.ejbt.2017.09.001&partnerID=40&md5=75279251f68e214b50155d13e05211b9
ISSN: 7173458
Appears in Collections:Scopus 1983-2021

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