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https://ir.swu.ac.th/jspui/handle/123456789/12994| Title: | Synergistic inhibition of human carcinoma cell growth via co-delivery of p53 plasmid DNA and bcl-2 antisense oligodeoxyribonucleotide by cholic acid-modified polyethylenimine |
| Authors: | Weecharangsan W. Opanasopit P. Niyomtham N. Yingyongnarongkul B.-E. Kewsuwan P. Lee R.J. |
| Keywords: | antisense oligodeoxynucleotide cholic acid green fluorescent protein plasmid DNA polyethyleneimine protein bcl 2 protein p53 antisense oligodeoxynucleotide BCL2 protein, human protein bcl 2 protein p53 agar gel electrophoresis antineoplastic activity Article cancer inhibition carcinoma cell cell culture cell growth controlled study fluorescence activated cell sorting genetic transfection growth inhibition human human cell nonviral gene delivery system particle size priority journal protein expression zeta potential antagonists and inhibitors carcinoma cell proliferation drug effects drug potentiation gene therapy gene vector genetics HeLa cell line metabolism pharmacology plasmid Carcinoma Cell Proliferation Drug Synergism Genetic Therapy Genetic Vectors Green Fluorescent Proteins HeLa Cells Humans Oligodeoxyribonucleotides, Antisense Plasmids Proto-Oncogene Proteins c-bcl-2 Tumor Suppressor Protein p53 |
| Issue Date: | 2017 |
| Abstract: | Background/Aim: This study investigated the codelivery of plasmid DNA and antisense oligodeoxyribonucleotide (AS ODN) into carcinoma cells by cholic acidmodified polyethylenimine (PEI-CA). Materials and Methods: PEI-CA/plasmid DNA and AS ODN complexes were formulated and evaluated for delivery of plasmid DNA and AS ODN in HeLa cells. The efficiency of co-delivery of plasmid DNA and AS ODN was evaluated by cell growth inhibition using p53 and bcl-2 AS ODN. Results: AS ODN intracellular delivery and green fluorescent protein expression upon cellular transfection were greater than in cells treated with uncomplexed nucleic acids. Treatment of the cells with PEI-CA/p53 plasmid DNA and bcl-2 AS ODN complexes resulted in cell growth inhibition that was greater than that of either PEI-CA/p53 plasmid DNA complexes or PEI-CA/bcl-2 AS ODN complexes alone. Conclusion: The co-delivery of p53 plasmid DNA and bcl-2 AS ODN in PEI-CA complexes enhanced therapeutic activities of both p53 plasmid DNA and bcl-2 AS ODN. |
| URI: | https://ir.swu.ac.th/jspui/handle/123456789/12994 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85032161426&doi=10.21873%2fanticanres.12085&partnerID=40&md5=6d730df18e244e989a66841bcef36e08 |
| ISSN: | 2507005 |
| Appears in Collections: | Scopus 1983-2021 |
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