Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/12810
ชื่อเรื่อง: Paper-based amperometric sensor for determination of acetylcholinesterase using screen-printed graphene electrode
ผู้แต่ง: Panraksa Y.
Siangproh W.
Khampieng T.
Chailapakul O.
Apilux A.
Keywords: Chemical detection
Chlorine compounds
Electrochemical electrodes
Graphene
Graphite electrodes
Spectrophotometry
Acetylcholinesterase
Amperometric detection
ELectrochemical detection
Graphene electrodes
Optimized conditions
Paper-based sensors
Screen-printed graphene electrodes
Spectro-photometric method
Amperometric sensors
acetylcholinesterase
acetylthiocholine
graphite
thiocholine
animal
chemistry
devices
electrochemistry
electrode
Electrophorus
enzyme assay
kinetics
metabolism
pH
printing
Acetylcholinesterase
Acetylthiocholine
Animals
Electrochemistry
Electrodes
Electrophorus
Enzyme Assays
Graphite
Hydrogen-Ion Concentration
Kinetics
Printing
Thiocholine
วันที่เผยแพร่: 2018
บทคัดย่อ: A simple paper-based sensor with electrochemical detection was first developed for rapid, selective, and sensitive determination of acetylcholinesterase (AChE). The screen-printed graphene electrode was used as working electrode providing sensitivity for the sensor. The amperometric detection of AChE is based on the determination of thiocholine (TCh) produced from hydrolysis of acetylthiocholine chloride (ATCh) by AChE. To detect TCh, the ATCh immobilized sheet was stacked onto the detection sheet using double adhesive tape, then samples of AChE were dropped on the back side of an ATCh immobilized sheet with only 1 min of incubation time. To avoid interference, glutathione (GSH), the potential of 0.5 V vs. Ag/AgCl was applied onto a graphene electrode and the current, which depends on AChE concentration, was measured. Under optimized conditions, the limit of detection (LOD) from the experiment of AChE determination was 0.1 U/mL with AChE concentration in range of 0.1–15 U/mL. The data correlated well with the data obtained using spectrophotometric method. The developed sensor had been successfully applied to detect AChE in blood samples. © 2017 Elsevier B.V.
URI: https://ir.swu.ac.th/jspui/handle/123456789/12810
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85033345539&doi=10.1016%2fj.talanta.2017.08.096&partnerID=40&md5=97aceab1fba4240a520726c21e31ea35
ISSN: 399140
Appears in Collections:Scopus 1983-2021

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