Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/12788
ชื่อเรื่อง: Egg-Hatching Mechanism of Human Liver Fluke, Opisthorchis viverrini: A Role for Leucine Aminopeptidases from the Snail Host, Bithynia siamensis goniomphalos
ผู้แต่ง: Khampoosa P.
Jones M.K.
Lovas E.M.
Piratae S.
Kulsuntiwong J.
Prasopdee S.
Srisawangwong T.
Laha T.
Sripanidkulchai B.
Thitapakorn V.
Tesana S.
Keywords: bestatin
cytosol aminopeptidase
cytosol aminopeptidase
enzyme inhibitor
leucine
protein phosphatase inhibitor-1
proteinase inhibitor
signal peptide
anoxic conditions
eggshell
enzyme
enzyme activity
host-parasite interaction
inhibition
inhibitor
intermediate disturbance hypothesis
invertebrate
parasite
snail
temperature effect
Article
Bithynia
Bithynia siamensis goniomphalos
cercaria
controlled study
digestive system
egg shell
electron microscopy
enzymatic degradation
ex vivo study
exocrine gland
food contamination
hatching
miracidium
nonhuman
Opisthorchis viverrini
pH
surface property
analysis of variance
animal
drug effect
enzymology
female
human
male
metabolism
Opisthorchis
ovum
parasitology
physiology
scanning electron microscopy
snail
transmission electron microscopy
ultrastructure
Bithynia
Digenea (flukes)
Fasciola hepatica
Gastropoda
Opisthorchis viverrini
Platyhelminthes
Sus
Trematoda
Analysis of Variance
Animals
Cercaria
Enzyme Inhibitors
Female
Humans
Intracellular Signaling Peptides and Proteins
Leucine
Leucyl Aminopeptidase
Male
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Opisthorchis
Ovum
Protease Inhibitors
Snails
วันที่เผยแพร่: 2018
บทคัดย่อ: The human liver fluke Opisthorchis viverrini (Platyhelminthes, Trematoda, Digenea) uses snails of the genus Bithynia as first intermediate host. Peculiarly among trematodes, the eggs of O. viverrini hatch within the digestive tract of its snail host. It remains uncertain whether hatching in this species is mediated through mechanical fracture of the eggshell or by digestion with specific digestive enzymes. This study aimed to characterize enzymes with specific inhibitors and factors involved in the hatching activity of O. viverrini eggs. For measuring egg hatching in vivo, 50 O. viverrini mature eggs were fed to individual Bithynia siamensis goniomphalos snails at various temperature conditions for 24 hr. Ex vivo, mature eggs were incubated with crude snail extract and commercial leucine aminopeptidase (LAP). Egg-hatching of O. viverrini was temperature dependent, with optimal hatching occurring at 24-28 C, with a peak of hatching of 93.54% in vivo and 30.55% ex vivo occurring at these temperatures. Ex vivo hatching rates increased to 45.87% under anaerobic conditions at 28 C. Some 22.70% and 16.21% of heat-killed eggs also hatched within the snail digestive tract and snail extract, respectively, indicating that host molecules are involved in the hatching response. Most eggs hatch in the anterior regions of the digestive tract. Hatching was completely inhibited in the presence of bestatin, an inhibitor of LAP, but not in the presence of phosphatase inhibitors. Bestatin inhibition of hatching was reversible. Finally, egg hatching could be induced by addition of a porcine LAP. The results indicate that this digenean utilizes both LAP of the snail host and movement of miracidia for hatching. © American Society of Parasitologists 2018.
URI: https://ir.swu.ac.th/jspui/handle/123456789/12788
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85048268748&doi=10.1645%2f16-125&partnerID=40&md5=1e4e3699d8ab990ba8232dd4845949cd
ISSN: 223395
Appears in Collections:Scopus 1983-2021

Files in This Item:
There are no files associated with this item.


Items in SWU repository are protected by copyright, with all rights reserved, unless otherwise indicated.