Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/12727
ชื่อเรื่อง: Potential of recombinant 2-Cys peroxiredoxin protein as a vaccine for Fasciola gigantica infection
ผู้แต่ง: Sangpairoj K.
Apisawetakan S.
Changklungmoa N.
Kueakhai P.
Chaichanasak P.
Sobhon P.
Chaithirayanon K.
Keywords: alanine aminotransferase
antibody
aspartate aminotransferase
Freund adjuvant
immunoglobulin G1
peroxiredoxin 2
alanine aminotransferase
aspartate aminotransferase
Freund adjuvant
helminth antibody
immunoglobulin G
peroxiredoxin
recombinant protein
vaccine
alanine aminotransferase blood level
animal cell
animal experiment
animal model
animal tissue
antibody blood level
Article
aspartate aminotransferase blood level
controlled study
drug efficacy
Fasciola gigantica
fascioliasis
female
humoral immunity
infection prevention
liver function
liver injury
metacercaria
mouse
nonhuman
priority journal
Th1 cell
Th2 cell
animal
blood
enzyme linked immunosorbent assay
enzymology
Fasciola
fascioliasis
immunology
Institute for Cancer Research mouse
liver
Lymnaea
parasitology
pathology
physiology
randomization
Alanine Transaminase
Animals
Antibodies, Helminth
Aspartate Aminotransferases
Enzyme-Linked Immunosorbent Assay
Fasciola
Fascioliasis
Female
Freund's Adjuvant
Immunoglobulin G
Liver
Lymnaea
Mice
Mice, Inbred ICR
Peroxiredoxins
Random Allocation
Recombinant Proteins
Vaccines
วันที่เผยแพร่: 2018
บทคัดย่อ: Helminth 2-cys peroxiredoxin (Prx) is a major antioxidant enzyme that protects parasites against hydrogen peroxide-generating oxidative stress from the hosts’ immune responses. This enzyme has been found in all stages of the tropical liver fluke, Fasciola gigantica. To investigate the potential of the recombinant F. gigantica Prx-2 (rFgPrx-2) as a vaccine candidate, vaccine trials in mice were carried out. In this study, the ICR mice were immunized with rFgPrx-2 combined with Freund's adjuvant and infected with F. gigantica metacercariae. The vaccine efficacy was estimated by quantitate fluke recovery, antibody levels and liver function. The protection by rFgPrx-2 against F. gigantica infection was achieved at 43–46% compared with adjuvant-infected and non-immunized-infected control groups, respectively. The vaccine elicited both Th1 and Th2 humoral immune responses with predominance of Th2 as indicated by the higher level of IgG1 in sera of immunized mice. However, the levels of liver damage markers, serum glutamate oxalic transaminase (SGOT) and serum glutamic pyruvate transaminase (SGPT) in rFgPrx-2 immunized group did not show significant difference in comparison with the controls. This study suggested that rFgPrx-2 may have a potential as a vaccine against tropical fasciolosis. © 2018
URI: https://ir.swu.ac.th/jspui/handle/123456789/12727
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85054808400&doi=10.1016%2fj.exppara.2018.09.005&partnerID=40&md5=3f571a66f3383896e347fcbd3b15ff2c
ISSN: 144894
Appears in Collections:Scopus 1983-2021

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