Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/12698
ชื่อเรื่อง: Nonformalin Fixative Agents: A Comparative Study of Fixative Efficacy and Histomorphology
ผู้แต่ง: Lam-ubol A.
Kitrueangphatchara K.
Putthanuparp T.
Arayakhun R.
Kwanthong R.
Choonhawarakorn K.
Keywords: alcohol
eosin
epinephrine
fixative
formaldehyde
hematoxylin
mepivacaine
alcohol
fixative
formaldehyde
mepivacaine
acantholysis
Article
basement membrane
cell junction
cell structure
comparative study
controlled study
epithelium cell
histomorphology
human
human cell
human tissue
inflammatory cell
intercellular space
mouth tissue
pathologist
priority journal
tissue fixation
tooth disease
artifact
chemistry
cytology
drug effect
mouth mucosa
pathology
procedures
staining
tissue fixation
tooth disease
tooth extraction
Artifacts
Epithelial Cells
Ethanol
Fixatives
Formaldehyde
Humans
Mepivacaine
Mouth Mucosa
Staining and Labeling
Tissue Fixation
Tooth Extraction
Tooth, Impacted
วันที่เผยแพร่: 2018
บทคัดย่อ: Objectives. Formalin is a gold standard fixative agent. However, formalin possesses health hazards and is not always available in rural areas. The objective of this study was to compare tissue fixative efficacy of nonformalin fixative agents with formalin. Methods. Oral tissues obtained during impacted tooth removal were collected. The tissue fragments were sectioned and fixed in 4 different fixatives; 30% jaggery, 70% ethanol, 2% mepivacaine with 1:100 000 epinephrine, or formalin for 24 and 72 hours. All specimens were then immersed in formalin for another 24 hours and processed according to standard protocol. Hematoxylin and eosin–stained sections were evaluated by a pathologist. The cellular structure, cellular outline, and quality of staining were graded from 1 to 3 and average fixative efficacy scores were compared using 1-way analysis of variance. Fixative artifacts were described. Results. Fixative efficacy scores of 70% ethanol and 30% jaggery at 24 and 72 hours were not statistically different from those of formalin. Conversely, 2% mepivacaine demonstrated significantly lower fixative efficacy scores than other agents. Although efficacy of each fixative was not statistically different between 24 and 72 hours, efficacy of 70% ethanol was markedly reduced at 72 hours when compared with others. Acantholysis of epithelial cells was the most notable artifact at 72 hours when fixed with nonformalin fixative agents. Conclusion. Both 70% ethanol and 30% jaggery provided acceptable fixative efficacy at 24 hours. However, only 30% jaggery maintained fixative efficacy at 72 hours. Nevertheless, negative effects on the epithelial cells were unavoidable and should be interpreted with caution. © The Author(s) 2018.
URI: https://ir.swu.ac.th/jspui/handle/123456789/12698
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85048089980&doi=10.1177%2f1066896918779450&partnerID=40&md5=035448097d7f0ff073fd065c574cd25d
ISSN: 10668969
Appears in Collections:Scopus 1983-2021

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