Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/12688
ชื่อเรื่อง: Cathepsin D in prawn reproductive system: its localization and function in actin degradation
ผู้แต่ง: Sukonset C.
Surinlert P.
Thongsum O.
Watthammawut A.
Somrit M.
Nakeim J.
Weerachatyanukul W.
Asuvapongpatana S.
Keywords: actin antibody
aldehyde
beta actin
cathepsin D
F actin
horseradish peroxidase
hydrogen peroxide
lysosome enzyme
paraformaldehyde
pepstatin
phalloidin
phosphate buffered saline
RNA directed DNA polymerase
acrosome
amino acid sequence
animal cell
animal model
animal tissue
Article
cell interaction
confocal microscopy
controlled study
densitometry
ejaculation
enzyme activity
epithelium cell
genetic transcription
genital system
glycosylation
housekeeping
human
human cell
hydrolysis
immunofluorescence
immunohistochemistry
in situ hybridization
Macrobrachium rosenbergii
nonhuman
phylogeny
protein fingerprinting
protein function
reverse transcription polymerase chain reaction
RNA extraction
SH-SY5Y cell line
somatic cell
spermatocyte
spermatogenesis
spermatogonium
sustentacular cell
testis
vas deferens
Western blotting
วันที่เผยแพร่: 2020
บทคัดย่อ: Cathepsin D (CAT-D) is a well-known aspartic protease that serves a function as housekeeping lysosomal enzyme in all somatic cells. Its existence in reproductive tissues is highly variable, even in the somatic derived epithelial cells of reproductive tract. In Macrobrachium rosenbergii, existence of MrCAT-D and its translational product was detected in both somatic cells (Sertoli-like supporting cells) and developing spermatogenic cells as well as along accessory spermatic ducts. Specifically, MrCAT-D was localized onto the sperm surface rather than within the acrosomal matrix, as evident by similar staining pattern of anti-CAT-D on live and aldehyde fixed sperm. MrCAT-D in testicular extracts and sperm isolates showed active enzyme activities towards its specific fluorogenic substrate (MCA-Gly-Lys-Pro-Ile-Leu-Phe-Phe-ArgLeu-Lys (Dnp)-D-Arg-NH2). MrCAT-D also exerted its function towards hydrolyzing filamentous actin, the meshwork of which is shown to be localized at the junction between germ cells and supporting cells and spermatogonia in M. rosenbergii testicular epithelium. Together, we have localized MrCAT-D transcript and its translational product in both supporting and germ cells of testis and claimed its enzymatic function towards actin degradation, which may be related to sperm release from the epithelial cell interaction. Copyright 2020 Sukonset et al.
URI: https://ir.swu.ac.th/jspui/handle/123456789/12688
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85095877062&doi=10.7717%2fpeerj.10218&partnerID=40&md5=bcad07659b8991bd731d2f98ad1d8f15
ISSN: 21678359
Appears in Collections:Scopus 1983-2021

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