Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/12624
ชื่อเรื่อง: Cost effective paper-based colorimetric devices for a simple assay of dopamine in pharmaceutical formulations using 3,3',5,5'-tetramethylbenzidine- silver nitrate as a chromogenic reagent
ผู้แต่ง: Manmana Y.
Chutvirasakul B.
Suntornsuk L.
Nuchtavorn N.
Keywords: dopamine
silver nitrate
tetramethylbenzidine
Article
assay
color
colorimetry
limit of detection
oxidation reduction reaction
reduction (chemistry)
ultraviolet spectrophotometry
วันที่เผยแพร่: 2019
บทคัดย่อ: Paper-based devices using smartphone-based detection have been widely used for colorimetric analysis due to their simplicity, ease of operation, and handheld capability. Herein, we demonstrate simple colorimetric measurement of dopamine on paper-based devices, which were fabricated in the 96-well plate shape using wax printing technique. The detection reaction was based on the 3,3',5,5'-tetramethylbenzidine (TMB)-silver nitrate redox reaction. The oxidized TMB possessed the blue color which could be observed by naked eyes. In the presence of dopamine, the blue color of the oxidized TMB decreased according to the reduction of the oxidized TMB. In optimal condition, the responses were fit to the logarithmic curve with r2 of 0.9932. The method showed good precision (%RSD < 6.6%), and recoveries (92.8 - 106.2%) with the detection limit of < 0.6 mM. Moreover, the results were well agreeing with those obtained from the conventional UV-Vis spectrophotometry, which were not statistically difference (P > 0.05). The developed method did not require any complex or time-consuming preparation and was successfully employed for the determination of dopamine injections. © Faculty of Pharmacy, Mahidol University (Thailand) 2018.
URI: https://ir.swu.ac.th/jspui/handle/123456789/12624
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85074728345&doi=10.29090%2fpsa.2019.04.018.0037&partnerID=40&md5=f862b24f5f64871ebf26c839e3d79bf5
ISSN: 25868195
Appears in Collections:Scopus 1983-2021

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