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https://ir.swu.ac.th/jspui/handle/123456789/12322| Title: | Generation of two human iPSC lines (MDCUi001-A and MDCUi001-B) from dermal fibroblasts of a Thai patient with X-linked osteogenesis imperfecta using integration-free Sendai virus |
| Authors: | Tongkobpetch S. Rungsiwiwut R. Pruksananonda K. Suphapeetiporn K. Shotelersuk V. |
| Keywords: | afp1 protein alpha smooth muscle actin beta tubulin cell marker genomic DNA glyceraldehyde 3 phosphate dehydrogenase kruppel like factor 4 membrane bound transcription factor peptidase site 2 Myc protein octamer transcription factor 4 peptidase rex1 protein tra 1 60 protein tra 1 81 protein transcription factor transcription factor NANOG transcription factor Sox2 unclassified drug kruppel like factor kruppel like factor 4 Myc protein MYC protein, human SOX2 protein, human transcription factor Sox Article cell clone cell differentiation cell reprogramming technique child controlled study ectoderm embryoid body endoderm human human cell in vitro study induced pluripotent stem cell karyotype male mdcui001 a cell line mdcui001 b cell line mesoderm nonhuman nuclear reprogramming osteogenesis imperfecta pluripotent stem cell line point mutation preschool child priority journal Sendai virus short tandem repeat skin fibroblast Thai (people) cell line cytology fibroblast genetics induced pluripotent stem cell metabolism osteogenesis imperfecta physiology Sendai virus Thailand Cell Differentiation Cell Line Fibroblasts Humans Induced Pluripotent Stem Cells Karyotype Kruppel-Like Transcription Factors Male Osteogenesis Imperfecta Proto-Oncogene Proteins c-myc Sendai virus SOXB1 Transcription Factors Thailand |
| Issue Date: | 2019 |
| Abstract: | Two clones of human induced pluripotent stem cells (iPSCs) were generated from dermal fibroblasts isolated from a one-year-old Thai patient with X-linked osteogenesis imperfecta. The patient harbored a mutation, p.N459S, in the MBTPS2 gene. The cells were reprogrammed using an integration-free Sendai virus containing KLF4, c-MYC, OCT4 and SOX2. Both of the established iPSC lines (MDCUi001-A and MDCUi001-B) maintained normal karyotype, expressed pluripotent markers and differentiated into all three germ layers. © 2019 |
| URI: | https://ir.swu.ac.th/jspui/handle/123456789/12322 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85069590379&doi=10.1016%2fj.scr.2019.101493&partnerID=40&md5=4e1ce5eee7a33b41e23cb8f86cf3a22a |
| ISSN: | 18735061 |
| Appears in Collections: | Scopus 1983-2021 |
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