Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/11976
ชื่อเรื่อง: Paper-based colorimetric biosensor of blood alcohol with in-situ headspace separation of ethanol from whole blood
ผู้แต่ง: Thepchuay Y.
Sonsa-ard T.
Ratanawimarnwong N.
Auparakkitanon S.
Sitanurak J.
Nacapricha D.
Keywords: Analytic equipment
Biosensors
Color
Colorimetry
Ethanol
Gas chromatography
Mammals
Mass spectrometry
Separation
ABTS
Blood alcohols
Colorimetric biosensors
Headspaces
Paper-based analytical devices
Whole blood
Blood
2,2' azinobis(3 ethylbenzothiazoline 6 sulfonic acid)
alcohol
alcohol oxidase
horseradish peroxidase
reagent
2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
alcohol
alcohol dehydrogenase
benzothiazole derivative
dyes, reagents, indicators, markers and buffers
sulfonic acid derivative
accuracy
Article
blood sampling
calibration
color
colorimetry
concentration (parameter)
diffusion
enzymatic assay
mass fragmentography
mouse
nonhuman
priority journal
reaction time
separation technique
sheep
validation process
animal
blood
chemistry
colorimetry
devices
enzymology
genetic procedures
horseradish
paper
procedures
reproducibility
Alcohol Oxidoreductases
Animals
Armoracia
Benzothiazoles
Biosensing Techniques
Colorimetry
Ethanol
Horseradish Peroxidase
Indicators and Reagents
Mice
Paper
Reproducibility of Results
Sulfonic Acids
วันที่เผยแพร่: 2020
บทคัดย่อ: This work presents a novel development that exploits the concept of in-situ gas-separation together with a specific enzymatic colorimetric detection to produce a portable biosensor called “Blood Alcohol Micro-pad” for direct quantitation of ethanol in whole blood. The thin square device (25 mm × 25 mm × 1.8 mm) comprises two layers of patterned filter paper held together with a double-sided mounting tape with an 8-mm circular hole (the headspace). In operation, the reagent is deposited on one layer and covered with sticky tape. Then 8 μL of a blood sample is dispensed onto the opposite layer and covered with sticky tape. Diffusion of ethanol across the 1.6 mm narrow headspace permits selective detection of ethanol by the enzymatic reagents deposited on the opposite layer. This reagent zone contains alcohol oxidase, horseradish peroxidase and 2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, as the chromogenic reagent. The color intensity, measured from the recorded digital image, resulting from the enzymatic assay of ethanol, correlates with the concentration of blood alcohol. The results obtained with spiked mice and sheep blood samples, using an external calibration in the range of 1–120 mg dL−1ethanol, gave recoveries of 93.2–104.4% (n = 12). The “Blood Alcohol Micro-pad” gave good precision with %RSD <1 (50 mg dL−1 ethanol, n = 10) and limit of quantification (10SD of intercept/slope) of 11.56 mg dL−1. The method was successfully validated against a headspace gas chromatography-mass spectrometric method. It has good potential for development as a simple and convenient blood alcohol sensor for on-site testing. © 2019 Elsevier B.V.
URI: https://ir.swu.ac.th/jspui/handle/123456789/11976
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85077544266&doi=10.1016%2fj.aca.2019.12.043&partnerID=40&md5=c25267876aa4c962a56e4d74336f1c2d
ISSN: 32670
Appears in Collections:Scopus 1983-2021

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