Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/11957
Full metadata record
DC FieldValueLanguage
dc.contributor.authorJitrakorn S.
dc.contributor.authorGangnonngiw W.
dc.contributor.authorBunnontae M.
dc.contributor.authorManajit O.
dc.contributor.authorRattanarojpong T.
dc.contributor.authorChaivisuthangkura P.
dc.contributor.authorDong H.T.
dc.contributor.authorSaksmerprome V.
dc.date.accessioned2021-04-05T03:01:32Z-
dc.date.available2021-04-05T03:01:32Z-
dc.date.issued2020
dc.identifier.issn448486
dc.identifier.other2-s2.0-85078548436
dc.identifier.urihttps://ir.swu.ac.th/jspui/handle/123456789/11957-
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85078548436&doi=10.1016%2fj.aquaculture.2020.734931&partnerID=40&md5=8c586418ab3a19934499877865d342e8
dc.description.abstractMegalocytivirus ISKNV and nervous necrosis virus (NNV) are two major viral pathogens affecting marine fish farms in Asia Pacific region. The present study reports an unexpected discovery of dual infections of both viruses in a single Asian sea bass (Lates calcarifer) farm experiencing ~50% cumulative fish mortality. A commercial Grunt Fin (GF) cell line was able to propagate concurrently two viruses from the clinical samples. Each virus was successfully purified from the co-infected cell culture, and its morphology was visualized by transmission electron microscopy (TEM). Subsequently, two viruses were identified as Megalocytivirus ISKNV and NNV from specific PCR assays using the extracted nucleic acids and sequencing of amplified PCR products. Approximately 104 and 103 copies of Megalocytivirus ISKNV and NNV were estimated in 200 ng DNA and RNA extracted from the co-infected cells. NNV propagation in GF cells was specifically confirmed using immunofluorescence microscopy. This is the first report for capability of NNV to propagate in the commercial GF cell line. This investigation opens up a promising in vitro model for studying concurrent infections of Megalocytivirus ISKNV and NNV and suggests a possible platform for concurrent production of Megalocytivirus ISKNV and NNV for bivalent vaccine. © 2020 Elsevier B.V.
dc.subjectcell
dc.subjectDNA
dc.subjectgenetic analysis
dc.subjectinfectivity
dc.subjectmortality
dc.subjectnervous system
dc.subjectperciform
dc.subjectpurification
dc.subjectRNA
dc.subjecttransmission electron microscopy
dc.subjectvirus
dc.subjectPacific Ocean
dc.subjectPacific Rim
dc.subjectAtlantic halibut nodavirus
dc.subjectLates calcarifer
dc.subjectMegalocytivirus
dc.subjectSerranidae
dc.subjectStriped Jack nervous necrosis virus
dc.titleInfectious cell culture system for concurrent propagation and purification of Megalocytivirus ISKNV and nervous necrosis virus from Asian Sea bass (Lates calcarifer)
dc.typeArticle
dc.rights.holderScopus
dc.identifier.bibliograpycitationAquaculture. Vol 520, (2020)
dc.identifier.doi10.1016/j.aquaculture.2020.734931
Appears in Collections:Scopus 1983-2021

Files in This Item:
There are no files associated with this item.


Items in SWU repository are protected by copyright, with all rights reserved, unless otherwise indicated.