Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/11808
Full metadata record
DC FieldValueLanguage
dc.contributor.authorOranratanaphan S.
dc.contributor.authorKengsakul M.
dc.contributor.authorTriratanachat S.
dc.contributor.authorKitkumthorn N.
dc.contributor.authorMutirangura A.
dc.contributor.authorTermrungruanglert W.
dc.date.accessioned2021-04-05T03:01:14Z-
dc.date.available2021-04-05T03:01:14Z-
dc.date.issued2020
dc.identifier.issn2476762X
dc.identifier.other2-s2.0-85094809714
dc.identifier.urihttps://ir.swu.ac.th/jspui/handle/123456789/11808-
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85094809714&doi=10.31557%2fAPJCP.2020.21.10.2913&partnerID=40&md5=b738492968d5d3baf1ac62da4362b526
dc.description.abstractBACKGROUND: Self sampled HPV testing is a cervical cancer screening method . However, cytology in self-sampled specimen cannot be used as a triage test.  Therefore, other methods for triage should be considered. CyclinA1 (CCNA1) promoter methylation has strong association with cervical precancerous and cancerous lesion. The objective of this study was to compare the diagnostic value of CCNA1 and self-sampled specimen for detecting high-grade cervical intraepithelial lesions or worse (CIN2+). MATERIALS AND METHODS: A cross sectional study was conducted. Women with abnormal cytology or positive for high risk HPV (hrHPV) indicated for colposcopic examination were enrolled.  Self-collected sampling for hrHPV DNA (SS-HPV) and CCNA1 were performed. hrHPV DNA testing was done by Cobas 4800 method. CCNA1 promoter methylation was detected by CCNA1 duplex methylation specific PCR. Histopathologic result as CIN2+ obtaining from colposcopic directed biopsy or excisional procedure  was considered as positive a gold standard. The results of hrHPV and CCNA1 were reported as positive or negative. Sensitivity specificity, positive predictive value, and negative predictive value of SS-HPV and CCNA1 were calculated by comparing the results with the gold standard. RESULTS: Two hundreds and eighty women were recruited. High-grade cervical lesions and cervical cancer (CIN2+) were diagnosed in 21.8% (61 cases) of the patients. The most common type of hrHPV was non 16, 18 subtype, followed by HPV16 and 18. CCNA1 was positive in 13 patients out of whom, twelve were CIN2+. Sensitivity of CCNA1 was 19.7 % and its  specificity and accuracy were 99.5% and 82.14%, respectively.  The sensitivity of SS-HPV was 70.5%, and its  specificity and accuracy were 39.2% and 43.3%, respectively. CONCLUSION:   Due to high specificity and positive predictive value of CCNA1, it can be used as alarming sign of having high-grade cervical intraepithelial lesions, especially in patient who has positive hrHPV DNA test based on self-collected sampling.<br />.
dc.titleCyclinA1 Promoter Methylation in Self-Sampling Test
dc.typeArticle
dc.rights.holderScopus
dc.identifier.bibliograpycitationAsian Pacific journal of cancer prevention : APJCP. Vol 21, No.10 (2020), p.2913-2917
dc.identifier.doi10.31557/APJCP.2020.21.10.2913
Appears in Collections:Scopus 1983-2021

Files in This Item:
There are no files associated with this item.


Items in SWU repository are protected by copyright, with all rights reserved, unless otherwise indicated.