dc.contributor.author |
Aunguldee T. |
|
dc.contributor.author |
Gerdprasert O. |
|
dc.contributor.author |
Tangteerawatana P. |
|
dc.contributor.author |
Jariyapongskul A. |
|
dc.contributor.author |
Leelayoova S. |
|
dc.contributor.author |
Wongsatayanon B.T. |
|
dc.date.accessioned |
2022-03-10T13:16:35Z |
|
dc.date.available |
2022-03-10T13:16:35Z |
|
dc.date.issued |
2021 |
|
dc.identifier.issn |
20366590 |
|
dc.identifier.other |
2-s2.0-85118305440 |
|
dc.identifier.uri |
https://ir.swu.ac.th/jspui/handle/123456789/17167 |
|
dc.identifier.uri |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85118305440&doi=10.3855%2fjidc.14472&partnerID=40&md5=3bdc637a8c61998d40e60096e2741d82 |
|
dc.description.abstract |
Introduction: In Thailand, Leishmania martiniquensis is the predominant species causing cutaneous and visceral leishmaniasis. Its incidence has been increasing among immunocompetent and immunocompromised hosts. We developed a prototype DNA vaccine using a partial consensus sequence of the cysteine protease B (cpb) gene derived from L. martiniquensis from Thai patients. Methodology: The laboratory inbred strain of albino BALB/c mice were immunized intramuscularly three times at 2-week intervals (weeks 0, 2, and 4) with cpb plasmid DNA (pcDNA_cpb) with or without the adjuvant, monoolein (pcDNA_cpb-MO). Mice were challenged at week 6 with L. martiniquensis promastigotes. Sera were analysed for IgG1, IgG2a, interferon gamma and interleukin 10 (IFN-γ and IL-10, respectively) levels at weeks 0, 4, and 9. Additionally, livers and spleens were also analysed for parasite burden using immunohistochemistry and real-time polymerase chain (qPCR) assays. Results: Three weeks after promastigote challenge, vaccinated mice showed significantly increased levels of IgG2a and IFN-γ while IL-10 level was significantly reduced when compared with those in the control group (p < 0.01). Parasite burden in the livers and spleens of vaccinated mice significantly decreased. In addition, a significant increase in mature granuloma formation in the livers when compared with those of the control group (p < 0.05) was found, indicating increased T-helper cells (Th1)-induced inflammation and destruction of amastigotes. Monoolein produced a booster effect to enhance the mouse Th1 protective immunity. Conclusions: The prototype DNA vaccine could induce a Th1 immune response that conferred potential protection to the L. martiniquensis promastigote challenge in BALB/c mice. © 2021 Aunguldee et al. |
|
dc.language |
en |
|
dc.subject |
cell protein |
|
dc.subject |
DNA vaccine |
|
dc.subject |
gamma interferon |
|
dc.subject |
glutamine |
|
dc.subject |
glycerol oleate |
|
dc.subject |
immunoglobulin G1 |
|
dc.subject |
immunoglobulin G2a |
|
dc.subject |
interleukin 10 |
|
dc.subject |
penicillin derivative |
|
dc.subject |
plasmid DNA |
|
dc.subject |
streptomycin |
|
dc.subject |
DNA vaccine |
|
dc.subject |
IL10 protein, human |
|
dc.subject |
interleukin 10 |
|
dc.subject |
animal experiment |
|
dc.subject |
animal model |
|
dc.subject |
animal tissue |
|
dc.subject |
antibody response |
|
dc.subject |
Article |
|
dc.subject |
controlled study |
|
dc.subject |
cpb gene |
|
dc.subject |
Escherichia coli |
|
dc.subject |
experimental inflammation |
|
dc.subject |
f Leishmania martiniquensis |
|
dc.subject |
gene |
|
dc.subject |
granuloma |
|
dc.subject |
immune response |
|
dc.subject |
immunization |
|
dc.subject |
immunofluorescence |
|
dc.subject |
immunogenicity |
|
dc.subject |
immunohistochemistry |
|
dc.subject |
inflammation |
|
dc.subject |
Leishmania |
|
dc.subject |
leishmaniasis |
|
dc.subject |
mouse |
|
dc.subject |
nonhuman |
|
dc.subject |
parasite load |
|
dc.subject |
promastigote |
|
dc.subject |
protein expression |
|
dc.subject |
real time polymerase chain reaction |
|
dc.subject |
recombinant plasmid |
|
dc.subject |
Th1 cell |
|
dc.subject |
animal |
|
dc.subject |
Bagg albino mouse |
|
dc.subject |
blood |
|
dc.subject |
cutaneous leishmaniasis |
|
dc.subject |
female |
|
dc.subject |
human |
|
dc.subject |
immunology |
|
dc.subject |
Leishmania |
|
dc.subject |
Thailand |
|
dc.subject |
vaccination |
|
dc.subject |
visceral leishmaniasis |
|
dc.subject |
Animals |
|
dc.subject |
Female |
|
dc.subject |
Humans |
|
dc.subject |
Interleukin-10 |
|
dc.subject |
Leishmania |
|
dc.subject |
Leishmaniasis, Cutaneous |
|
dc.subject |
Leishmaniasis, Visceral |
|
dc.subject |
Mice |
|
dc.subject |
Mice, Inbred BALB C |
|
dc.subject |
Thailand |
|
dc.subject |
Vaccination |
|
dc.subject |
Vaccines, DNA |
|
dc.title |
Immunogenicity and potential protection of DNA vaccine of Leishmania martiniquensis against Leishmania infection in mice |
|
dc.type |
Article |
|
dc.rights.holder |
Scopus |
|
dc.identifier.bibliograpycitation |
Journal of Infection in Developing Countries. Vol 15, No.9 (2021), p.328-1338 |
|
dc.identifier.doi |
10.3855/jidc.14472 |
|