dc.contributor.author |
Sithigorngul W. |
|
dc.contributor.author |
Jaideechoey S. |
|
dc.contributor.author |
Saraithongkum W. |
|
dc.contributor.author |
Longyant S. |
|
dc.contributor.author |
Sithigorngul P. |
|
dc.date.accessioned |
2021-04-05T04:33:30Z |
|
dc.date.available |
2021-04-05T04:33:30Z |
|
dc.date.issued |
1999 |
|
dc.identifier.issn |
0022104X |
|
dc.identifier.other |
2-s2.0-0033168282 |
|
dc.identifier.uri |
https://ir.swu.ac.th/jspui/handle/123456789/15312 |
|
dc.identifier.uri |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033168282&doi=10.1002%2f%28SICI%291097-010X%2819990701%29284%3a2%3c217%3a%3aAID-JEZ11%3e3.0.CO%3b2-5&partnerID=40&md5=ae0dca1a21c14f5eb233bda7e5db2573 |
|
dc.description.abstract |
Isolation of the crustacean hyperglycemic hormone (CHH) from the eyestalk of the giant freshwater prawn Macrobrachium rosenbergii was performed using 5,000 ground eye-stalks extracted in methanol-acetic acid-water (90:1:9). After the extract was partially purified using C18 cartridges, it was further purified by eight steps of RP-HPLC using four kinds of columns: C18, C8, cyano and phenyl, and three solvent systems: acetonitrile (ACN)/trifluoroacetic acid, ACN/heptafluoroacetic acid and ACN/triethylammonium acetate. The bioassay of CHH during purification was done by injection of eluate fractions into eyestalk-ablated prawns and determination of the ability of the fractions to elevate glucose in the haemolymph. A complete amino acid sequence analysis was performed on one isoform of CHH (Mar-CHH-1), consisting of 71 residues. The sequence of Mar-CHH-1 shows considerable similarity (45-68%) to CHHs reported in other crustaceans. It is expected that there might be more than one isoform of CHH in M. rosenbergii. |
|
dc.subject |
acetonitrile |
|
dc.subject |
amino acid sequence |
|
dc.subject |
bioassay |
|
dc.subject |
crustacean hyperglycemic hormone |
|
dc.subject |
Macrobrachium rosenbergii |
|
dc.subject |
purification |
|
dc.subject |
reversed phase high performance liquid chromatography |
|
dc.subject |
solvent extraction |
|
dc.subject |
trifluoroacetic acid |
|
dc.subject |
Amino Acid Sequence |
|
dc.subject |
Animals |
|
dc.subject |
Chromatography, High Pressure Liquid |
|
dc.subject |
Eye |
|
dc.subject |
Female |
|
dc.subject |
Ganglia, Invertebrate |
|
dc.subject |
Glucose |
|
dc.subject |
Hemolymph |
|
dc.subject |
Hyperglycemia |
|
dc.subject |
Invertebrate Hormones |
|
dc.subject |
Molecular Sequence Data |
|
dc.subject |
Palaemonidae |
|
dc.subject |
Protein Isoforms |
|
dc.subject |
Sequence Homology, Amino Acid |
|
dc.subject |
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
|
dc.title |
Purification and characterization of an isoform of crustacean hyperglycemic hormone from the eyestalk of Macrobrachium rosenbergii |
|
dc.type |
Article |
|
dc.rights.holder |
Scopus |
|
dc.identifier.bibliograpycitation |
Journal of Experimental Zoology. Vol 284, No.2 (1999), p.217-224 |
|
dc.identifier.doi |
10.1002/(SICI)1097-010X(19990701)284:2<217 |
|