dc.contributor.author |
Deachapunya C. |
|
dc.contributor.author |
Poonyachoti S. |
|
dc.contributor.author |
Krishnamra N. |
|
dc.date.accessioned |
2021-04-05T04:31:59Z |
|
dc.date.available |
2021-04-05T04:31:59Z |
|
dc.date.issued |
2008 |
|
dc.identifier.issn |
220795 |
|
dc.identifier.other |
2-s2.0-44949171494 |
|
dc.identifier.uri |
https://ir.swu.ac.th/jspui/handle/123456789/14852 |
|
dc.identifier.uri |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-44949171494&doi=10.1677%2fJOE-08-0077&partnerID=40&md5=3625f22db008d6610e16cfee31bde40b |
|
dc.description.abstract |
The effect of prolactin (PRL) onion transport across the porcine glandular endometrial epithelial cells was studied in primary cell culture using the short-circuit current technique. Addition of 1 μg/ml PRL either to the apical solution or to the basolateral solution produced a peak followed by a sustained increase in Isc, but with a lesser response when PRL was added apically. Basolateral addition of PRL increased the Isc in a concentration-dependent manner with a maximum effiect at 1 μg/ml and an effective concentration value of 120 ng/ml. The PRL-stimulated Isc was significantly reduced by pretreatment with an apical addition of 5-nitro-2-(3-phenylpropylamino) benzoic acid (200 μM), diphenylamine-2-carboxylic acid (1 mM) or 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid (200 μM), Cl- channel blockers, but not by amiloride (10 μM), a Na+ channel blocker. In addition, pretreatment with bumetanide (200 μM), a Na + -K + -2Cl- cotransporter inhibitor, in the basolateral solution significantly reduced the PRL-stimulated Isc. Replacement of Cl- or HCO3- in the bathing solutions also decreased the Isc response to PRL. Pretreatment of the monolayer with AG490 (50 μM), an inhibitor of JAK2 activity significantly inhibited the PRL-induced increase in Isc. Western blot analysis of the porcine endometrial epithelial cells revealed the presence of short isoform of PRL receptor (PRLR-S) that could be regulated by 17β-estradiol. The results of this investigation showed that PRL acutely stimulated anion secretion across the porcine endometrial epithelial cells possibly through PRLR-S present in both apical and basolateral membranes. The PRL response appeared to be mediated by the JAK2-dependent pathway. © 2008 Society for Endocrinology. |
|
dc.subject |
4,4' diisothiocyanatostilbene 2,2' disulfonic acid |
|
dc.subject |
5 nitro 2 (3 phenylpropylamino)benzoic acid |
|
dc.subject |
amiloride |
|
dc.subject |
bumetanide |
|
dc.subject |
chloride channel blocking agent |
|
dc.subject |
efenamic acid |
|
dc.subject |
estradiol |
|
dc.subject |
Janus kinase 2 |
|
dc.subject |
loop diuretic agent |
|
dc.subject |
n benzyl 2 cyano 3 (3,4 dihydroxyphenyl)acrylamide |
|
dc.subject |
prolactin |
|
dc.subject |
prolactin receptor |
|
dc.subject |
sodium channel blocking agent |
|
dc.subject |
animal cell |
|
dc.subject |
animal tissue |
|
dc.subject |
article |
|
dc.subject |
cell isolation |
|
dc.subject |
cell stimulation |
|
dc.subject |
concentration response |
|
dc.subject |
controlled study |
|
dc.subject |
culture medium |
|
dc.subject |
electrolyte transport |
|
dc.subject |
endometrium |
|
dc.subject |
enzyme activity |
|
dc.subject |
epithelium cell |
|
dc.subject |
female |
|
dc.subject |
nonhuman |
|
dc.subject |
priority journal |
|
dc.subject |
protein expression |
|
dc.subject |
short circuit current |
|
dc.subject |
swine |
|
dc.subject |
Western blotting |
|
dc.subject |
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid |
|
dc.subject |
Animals |
|
dc.subject |
Bicarbonates |
|
dc.subject |
Chlorides |
|
dc.subject |
Electrolytes |
|
dc.subject |
Endometrium |
|
dc.subject |
Epithelial Cells |
|
dc.subject |
Female |
|
dc.subject |
Ion Transport |
|
dc.subject |
Janus Kinase 2 |
|
dc.subject |
Nitrobenzoates |
|
dc.subject |
Potassium |
|
dc.subject |
Prolactin |
|
dc.subject |
Receptors, Prolactin |
|
dc.subject |
Signal Transduction |
|
dc.subject |
Sodium |
|
dc.subject |
Swine |
|
dc.title |
Regulation of electrolyte transport across cultured endometrial epithelial cells by prolactin |
|
dc.type |
Article |
|
dc.rights.holder |
Scopus |
|
dc.identifier.bibliograpycitation |
Journal of Endocrinology. Vol 197, No.3 (2008), p.575-582 |
|
dc.identifier.doi |
10.1677/JOE-08-0077 |
|