dc.contributor.author |
Deachapunya C. |
|
dc.contributor.author |
Poonyachoti S. |
|
dc.contributor.author |
Krishnamra N. |
|
dc.date.accessioned |
2021-04-05T03:34:17Z |
|
dc.date.available |
2021-04-05T03:34:17Z |
|
dc.date.issued |
2012 |
|
dc.identifier.issn |
1931857 |
|
dc.identifier.other |
2-s2.0-84861141161 |
|
dc.identifier.uri |
https://ir.swu.ac.th/jspui/handle/123456789/14340 |
|
dc.identifier.uri |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84861141161&doi=10.1152%2fajpgi.00143.2011&partnerID=40&md5=d81c03736704ab3359dde25464f821d6 |
|
dc.description.abstract |
The effect of prolactin (PRL) on ion transport across the rat colon epithelium was investigated using Ussing chamber technique. PRL (1 μg/ml) induced a sustained decrease in short-circuit current (I sc) in the distal colon with an EC 50 value of 100 ng/ml and increased I sc in the proximal colon with an EC 50 value of 49 ng/ml. In the distal colon, the PRL-induced decrease in I sc was not affected by Na + channel blocker amiloride or Cl - channel blockers, NPPB, DPC, or DIDS, added mucosally. However, the response was inhibited by mucosal application of K + channel blockers glibenclamide, quinidine, and chromanol 293B, whereas other K + channel blockers, Ba 2+, tetraethylammonium, clotrimazole, and apamin, failed to have effects. The PRL-induced decrease in I sc was also inhibited by Na +-K +-2Cl - transporter inhibitor bumetanide, Ba 2+, and chromanol 293B applied serosally. In the transverse and proximal colon, the PRL-induced increase in I sc was suppressed by DPC, glibenclamide, and bumetanide, but not by NPPB, DIDS, or amiloride. The PRL-induced changes in I sc in both distal and proximal colon were abolished by JAK2 inhibitor AG490, but not BAPTA-AM, the Ca 2+ chelating agent, or phosphatidylinositol 3-kinase inhibitor wortmannin. These results suggest a segment-specific effect of PRL in rat colon, by activation of K + secretion in the distal colon and activation of Cl - secretion in the transverse and proximal colon. Both PRL actions are mediated by JAK-STAT-dependent pathway, but not phosphatidylinositol 3-kinase pathway or Ca 2+ mobilization. These findings suggest a role of PRL in the regulation of electrolyte transport in mammalian colon. © 2012 the American Physiological Society. |
|
dc.subject |
amiloride |
|
dc.subject |
apamin |
|
dc.subject |
bumetanide |
|
dc.subject |
carbachol |
|
dc.subject |
chromanol 293B |
|
dc.subject |
clotrimazole |
|
dc.subject |
ethylene glycol 1,2 bis(2 aminophenyl) ether n,n,n',n' tetraacetic acid |
|
dc.subject |
glibenclamide |
|
dc.subject |
isobutylmethylxanthine |
|
dc.subject |
n benzyl 2 cyano 3 (3,4 dihydroxyphenyl)acrylamide |
|
dc.subject |
phosphatidylinositol 3 kinase |
|
dc.subject |
prolactin |
|
dc.subject |
quinidine |
|
dc.subject |
sodium potassium chloride cotransporter |
|
dc.subject |
tetrylammonium |
|
dc.subject |
wortmannin |
|
dc.subject |
animal experiment |
|
dc.subject |
animal tissue |
|
dc.subject |
article |
|
dc.subject |
ascending colon |
|
dc.subject |
calcium mobilization |
|
dc.subject |
colon mucosa |
|
dc.subject |
concentration response |
|
dc.subject |
controlled study |
|
dc.subject |
descending colon |
|
dc.subject |
electrolyte transport |
|
dc.subject |
electrophysiology |
|
dc.subject |
ion transport |
|
dc.subject |
male |
|
dc.subject |
nonhuman |
|
dc.subject |
priority journal |
|
dc.subject |
rat |
|
dc.subject |
signal transduction |
|
dc.subject |
sodium absorption |
|
dc.subject |
transverse colon |
|
dc.subject |
Animals |
|
dc.subject |
Chloride Channels |
|
dc.subject |
Chlorides |
|
dc.subject |
Colon |
|
dc.subject |
Enzyme Inhibitors |
|
dc.subject |
Intestinal Mucosa |
|
dc.subject |
Ion Transport |
|
dc.subject |
Male |
|
dc.subject |
MAP Kinase Signaling System |
|
dc.subject |
Potassium |
|
dc.subject |
Potassium Channel Blockers |
|
dc.subject |
Prolactin |
|
dc.subject |
Rats |
|
dc.subject |
Rats, Wistar |
|
dc.subject |
Sodium Channel Blockers |
|
dc.subject |
Tyrphostins |
|
dc.title |
Site-specific regulation of ion transport by prolactin in rat colon epithelium |
|
dc.type |
Article |
|
dc.rights.holder |
Scopus |
|
dc.identifier.bibliograpycitation |
American Journal of Physiology - Gastrointestinal and Liver Physiology. Vol 302, No.10 (2012), p.G1199-G1206 |
|
dc.identifier.doi |
10.1152/ajpgi.00143.2011 |
|