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Interphase-FISH screening for eight common rearrangements in pediatric B-cell precursor acute lymphoblastic leukemia

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dc.contributor.author Hutspardol S.
dc.contributor.author Pakakasama S.
dc.contributor.author Kanta K.
dc.contributor.author Nuntakarn L.
dc.contributor.author Anurathapan U.
dc.contributor.author Sirachainan N.
dc.contributor.author Songdej D.
dc.contributor.author Sawangpanich R.
dc.contributor.author Tiyasirichokchai R.
dc.contributor.author Rerkamnuaychoke B.
dc.contributor.author Hongeng S.
dc.date.accessioned 2021-04-05T03:32:53Z
dc.date.available 2021-04-05T03:32:53Z
dc.date.issued 2013
dc.identifier.issn 17515521
dc.identifier.other 2-s2.0-84880621984
dc.identifier.uri https://ir.swu.ac.th/jspui/handle/123456789/14027
dc.identifier.uri https://www.scopus.com/inward/record.uri?eid=2-s2.0-84880621984&doi=10.1111%2fijlh.12031&partnerID=40&md5=f204742139e63a8d1787436558acbd4b
dc.description.abstract Summary: Introduction: This is the first pilot study to screen multiple common genetic aberrations in B-cell precursor acute lymphoblastic leukemia (BCP-ALL). Methods: Thirty-two children with BCP-ALL were investigated for chromosomal rearrangements using interphase fluorescence in situ hybridization (FISH). Eight common translocations and rearrangements, including ETV6-RUNX1, TCF3-PBX1, BCR-ABL1, ETV6, TCF3, MLL, IGH@, and PAX5, were tested for using dual-color DNA probes. Results: ETV6-RUNX1 was the most frequent translocation detected in 11 children (34.4%). Two patients with BCR-ABL1 (6.3%) and one with TCF3-PBX1 (3.1%) translocations were also observed. Using break-apart probes, 11 children (34.4%) had a positive FISH result for ETV6, two patients for IGH@ (6.3%), one patient for MLL (3.1%), and one patient for PAX5 rearrangements (3.1%). All patients with the ETV6-RUNX1 fusion were also identified by split signals for ETV6. Other abnormalities, including extra copies and deletion of genes, were observed within the range of 3.1-34.4%. Cytogenetics analysis showed a single case each of BCR-ABL1 fusion, MLL, and IGH@ rearrangements (3.1% each). ETV6-RUNX1 fusion and ETV6 split-apart rearrangements were not visible by cytogenetics. Likewise, one each of cases with TCF3-PBX1 fusion and with PAX5 split signal seen by FISH was not visible by cytogenetics. Conclusion: By using 8 FISH probes in conjunction cytogenetics for the detection of common aberrations, interphase FISH enhanced the detection of chromosomal rearrangements in children with BCP-ALL. © 2012 John Wiley & Sons Ltd.
dc.subject Abelson kinase
dc.subject breakpoint cluster region protein
dc.subject immunoglobulin heavy chain
dc.subject mixed lineage leukemia protein
dc.subject transcription factor 7 like 1
dc.subject transcription factor ETV6
dc.subject transcription factor PAX5
dc.subject transcription factor PBX1
dc.subject transcription factor RUNX1
dc.subject acute lymphoblastic leukemia
dc.subject article
dc.subject child
dc.subject chromosome rearrangement
dc.subject clinical article
dc.subject cytogenetics
dc.subject DNA probe
dc.subject female
dc.subject fluorescence in situ hybridization
dc.subject fusion gene
dc.subject gene deletion
dc.subject gene translocation
dc.subject human
dc.subject infant
dc.subject interphase
dc.subject male
dc.subject pre B lymphocyte
dc.subject preschool child
dc.subject priority journal
dc.subject school child
dc.subject screening
dc.subject ALL
dc.subject B-cells
dc.subject FISH
dc.subject Acute Disease
dc.subject Adolescent
dc.subject B-Lymphocytes
dc.subject Child
dc.subject Child, Preschool
dc.subject Female
dc.subject Genetic Testing
dc.subject Humans
dc.subject In Situ Hybridization, Fluorescence
dc.subject Infant
dc.subject Interphase
dc.subject Karyotyping
dc.subject Male
dc.subject Oncogene Proteins, Fusion
dc.subject Pilot Projects
dc.subject Precursor B-Cell Lymphoblastic Leukemia-Lymphoma
dc.subject Translocation, Genetic
dc.title Interphase-FISH screening for eight common rearrangements in pediatric B-cell precursor acute lymphoblastic leukemia
dc.type Article
dc.rights.holder Scopus
dc.identifier.bibliograpycitation International Journal of Laboratory Hematology. Vol 35, No.4 (2013), p.406-415
dc.identifier.doi 10.1111/ijlh.12031


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