dc.contributor.author |
Plaon S. |
|
dc.contributor.author |
Longyant S. |
|
dc.contributor.author |
Sithigorngul P. |
|
dc.contributor.author |
Chaivisuthangkura P. |
|
dc.date.accessioned |
2021-04-05T03:26:25Z |
|
dc.date.available |
2021-04-05T03:26:25Z |
|
dc.date.issued |
2015 |
|
dc.identifier.issn |
8997659 |
|
dc.identifier.other |
2-s2.0-84940045331 |
|
dc.identifier.uri |
https://ir.swu.ac.th/jspui/handle/123456789/13779 |
|
dc.identifier.uri |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84940045331&doi=10.1080%2f08997659.2015.1037468&partnerID=40&md5=bf42cede0883a05711c8c7de7b78d58e |
|
dc.description.abstract |
Vibrio alginolyticus is a major bacterial pathogen causing disease in marine animals. The present study aimed to develop a loop-mediated isothermal amplification (LAMP) coupled with a lateral flow dipstick (LFD) for rapid and simple visual detection of V. alginolyticus–specific amplicons. The biotin-labeled LAMP amplicons from the targeted portion of a gene encoding rpoS-like sigma factor (rpoX) were generated at 60°C for 1 h and then hybridized with a fluorescein isothiocyanate–labeled probe for 5 min for visual detection with LFD. In pure cultures, the detection limit of the LAMP–LFD technique for V. alginolyticus was 1.8 × 102 CFU/mL while that of PCR was 1.8 × 103 CFU/mL. In spiked whiteleg shrimp samples Penaeus vannamei, the sensitivity for V. alginolyticus detection was 2 × 103 CFU/g (equivalent to 4 CFU per reaction) while PCR was 10 times less sensitive. The LAMP–LFD method for V. alginolyticus correctly identified 21 isolates of V. alginolyticus but did not recognize 23 non-V. alginolyticus Vibrio isolates and 15 non-Vibrio isolates. In summary, this LAMP–LFD method targeted to the rpoX gene is a convenient assay for specific identification of V. alginolyticus with high sensitivity. © American Fisheries Society 2015. |
|
dc.subject |
Animalia |
|
dc.subject |
Bacteria (microorganisms) |
|
dc.subject |
Litopenaeus vannamei |
|
dc.subject |
Vibrio |
|
dc.subject |
Vibrio alginolyticus |
|
dc.subject |
bacterial protein |
|
dc.subject |
molecular probe |
|
dc.subject |
chemistry |
|
dc.subject |
gene expression regulation |
|
dc.subject |
genetics |
|
dc.subject |
isolation and purification |
|
dc.subject |
metabolism |
|
dc.subject |
molecular probe |
|
dc.subject |
nucleic acid amplification |
|
dc.subject |
physiology |
|
dc.subject |
procedures |
|
dc.subject |
Vibrio alginolyticus |
|
dc.subject |
Bacterial Proteins |
|
dc.subject |
Gene Expression Regulation, Bacterial |
|
dc.subject |
Molecular Probes |
|
dc.subject |
Nucleic Acid Amplification Techniques |
|
dc.subject |
Vibrio alginolyticus |
|
dc.title |
Rapid and sensitive detection of Vibrio alginolyticus by loop-mediated isothermal amplification combined with a lateral flow dipstick targeted to the rpoX gene |
|
dc.type |
Article |
|
dc.rights.holder |
Scopus |
|
dc.identifier.bibliograpycitation |
Journal of Aquatic Animal Health. Vol 27, No.3 (2015), p.156-163 |
|
dc.identifier.doi |
10.1080/08997659.2015.1037468 |
|