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Production, purification and characterization of an ionic liquid tolerant cellulase from Bacillus sp. Isolated from rice paddy field soil

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dc.contributor.author Sriariyanun M.
dc.contributor.author Tantayotai P.
dc.contributor.author Yasurin P.
dc.contributor.author Pornwongthong P.
dc.contributor.author Cheenkachorn K.
dc.date.accessioned 2021-04-05T03:24:18Z
dc.date.available 2021-04-05T03:24:18Z
dc.date.issued 2016
dc.identifier.issn 7173458
dc.identifier.other 2-s2.0-84954547807
dc.identifier.uri https://ir.swu.ac.th/jspui/handle/123456789/13496
dc.identifier.uri https://www.scopus.com/inward/record.uri?eid=2-s2.0-84954547807&doi=10.1016%2fj.ejbt.2015.11.002&partnerID=40&md5=2e7518ed5e3f659a582c51e05c2d779e
dc.description.abstract Background: Lignocellulosic biomass is a renewable, abundant, and inexpensive resource for biorefining process to produce biofuel and valuable chemicals. To make the process become feasible, it requires the use of both efficient pretreatment and hydrolysis enzymes to generate fermentable sugars. Ionic liquid (IL) pretreatment has been demonstrated to be a promising method to enhance the saccharification of biomass by cellulase enzyme; however, the remaining IL in the hydrolysis buffer strongly inhibits the function of cellulase. This study aimed to isolate a potential IL-tolerant cellulase producing bacterium to be applied in biorefining process. Result: One Bacillus sp., MSL2 strain, obtained from rice paddy field soil was isolated based on screening of cellulase assay. Its cellulase enzyme was purified and fractionated using a size exclusion chromatography. The molecular weight of purified cellulose was 48 kDa as revealed by SDS-PAGE and zymogram analysis. In the presence of the IL, 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]) concentration of 1 M, the cellulase activity retained 77.7% of non-IL condition. In addition, the optimum temperature and pH of the enzyme is 50°C and pH 6.0, respectively. However, this cellulase retained its activity more than 90% at 55°C, and pH 4.0. Kinetic analysis of purified enzyme showed that the Km and Vmax were 0.8 mg/mL and 1000 μM/min, respectively. Conclusion: The characterization of cellulase produced from MSL2 strain was described here. These properties of cellulase made this bacterial strain become potential to be used in the biorefining process. © 2015 Pontificia Universidad Católica de Valparaíso.
dc.subject Bacteriology
dc.subject Chromatography
dc.subject Enzymes
dc.subject Hydrolysis
dc.subject Ionic liquids
dc.subject Liquids
dc.subject Purification
dc.subject Refining
dc.subject Saccharification
dc.subject Size exclusion chromatography
dc.subject 1-ethyl-3-methylimidazolium acetates
dc.subject Biorefineries
dc.subject Cellulase
dc.subject Cellulase activity
dc.subject Fermentable sugars
dc.subject Lignocellulosic biomass
dc.subject Optimum temperature
dc.subject Valuable chemicals
dc.subject Biomass
dc.subject 1 ethyl 3 methylimidazolium acetate
dc.subject cellulase
dc.subject imidazole derivative
dc.subject ionic liquid
dc.subject unclassified drug
dc.subject Article
dc.subject Bacillus
dc.subject bacterial strain
dc.subject concentration (parameters)
dc.subject controlled study
dc.subject enzyme activity
dc.subject enzyme analysis
dc.subject enzyme purification
dc.subject enzyme synthesis
dc.subject molecular weight
dc.subject nonhuman
dc.subject pH
dc.subject protein hydrolysis
dc.subject size exclusion chromatography
dc.subject soil
dc.subject temperature
dc.subject zymography
dc.title Production, purification and characterization of an ionic liquid tolerant cellulase from Bacillus sp. Isolated from rice paddy field soil
dc.type Article
dc.rights.holder Scopus
dc.identifier.bibliograpycitation Electronic Journal of Biotechnology. Vol 19, No.1 (2016), p.23-28
dc.identifier.doi 10.1016/j.ejbt.2015.11.002


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