dc.contributor.author |
Wachiralurpan S. |
|
dc.contributor.author |
Sriyapai T. |
|
dc.contributor.author |
Areekit S. |
|
dc.contributor.author |
Sriyapai P. |
|
dc.contributor.author |
Thongphueak D. |
|
dc.contributor.author |
Santiwatanakul S. |
|
dc.contributor.author |
Chansiri K. |
|
dc.date.accessioned |
2021-04-05T03:21:55Z |
|
dc.date.available |
2021-04-05T03:21:55Z |
|
dc.date.issued |
2017 |
|
dc.identifier.issn |
17599660 |
|
dc.identifier.other |
2-s2.0-85035064952 |
|
dc.identifier.uri |
https://ir.swu.ac.th/jspui/handle/123456789/12967 |
|
dc.identifier.uri |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85035064952&doi=10.1039%2fc7ay01750b&partnerID=40&md5=fdca4d28bf6be653e60d2b3cad8e3b97 |
|
dc.description.abstract |
A rapid and specific, hly-based, loop-mediated isothermal amplification (LAMP) was applied for the detection of Listeria monocytogenes in food and food products, using a real-time turbidimeter platform (LAMP-turbidity). The principle behind this method relies on an increase in a DNA yield, which correlates with the production of magnesium pyrophosphate, and the results can be determined via an amplification curve within 1 h. The specificity test revealed that L. monocytogenes (DMST 17303) was observed from 34.1 to 38.3 min, while thirty strains of non-L. monocytogenes demonstrated no cross-reactions. The limits of detection for purified genomic DNA and pure culture were 800 pg μL-1 and 2.82 × 103 CFU mL-1, respectively. Investigation on 200 raw chicken meat samples indicated that the specificity, sensitivity, and accuracy of LAMP-turbidity were 100%, 62.75%, and 90.50%, respectively. These data suggest that an hly-based, real-time, quantitative LAMP-turbidity assay can be an applicable tool for the epidemiological screening of L. monocytogenes in food and food products. © 2017 The Royal Society of Chemistry. |
|
dc.subject |
Antigen-antibody reactions |
|
dc.subject |
Diseases |
|
dc.subject |
Food products |
|
dc.subject |
Isotherms |
|
dc.subject |
Listeria |
|
dc.subject |
Screening |
|
dc.subject |
Turbidity |
|
dc.subject |
Amplification curves |
|
dc.subject |
Cross reaction |
|
dc.subject |
Limits of detection |
|
dc.subject |
Listeria monocytogenes |
|
dc.subject |
Loop mediated isothermal amplifications |
|
dc.subject |
Monocytogenes |
|
dc.subject |
Rapid screening tests |
|
dc.subject |
Real-time turbidimeters |
|
dc.subject |
Food microbiology |
|
dc.title |
A one-step rapid screening test of: Listeria monocytogenes in food samples using a real-time loop-mediated isothermal amplification turbidity assay |
|
dc.type |
Article |
|
dc.rights.holder |
Scopus |
|
dc.identifier.bibliograpycitation |
Analytical Methods. Vol 9, No.45 (2017), p.6403-6410 |
|
dc.identifier.doi |
10.1039/c7ay01750b |
|