dc.contributor.author |
Chutvirasakul B. |
|
dc.contributor.author |
Nuchtavorn N. |
|
dc.contributor.author |
Suntornsuk L. |
|
dc.contributor.author |
Zeng Y. |
|
dc.date.accessioned |
2021-04-05T03:01:35Z |
|
dc.date.available |
2021-04-05T03:01:35Z |
|
dc.date.issued |
2020 |
|
dc.identifier.issn |
1730835 |
|
dc.identifier.other |
2-s2.0-85078672314 |
|
dc.identifier.uri |
https://ir.swu.ac.th/jspui/handle/123456789/11995 |
|
dc.identifier.uri |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85078672314&doi=10.1002%2felps.201900323&partnerID=40&md5=e7dd10d56865f67e2b511fea899b78a8 |
|
dc.description.abstract |
Exosome quantification is important for estimation of informative messengers (e.g., proteins, lipids, RNA, etc.) involving physiological and pathological effects. This work aimed to develop a simple and rapid distance-based paper portable device using exosome-capture vesicles (polydiacetylene conjugated with antiCD81) for exosome quantification in cell cultures. This novel concept relied on distinct aggregation of exosomes and exosome-capture vesicles leading to different solvent migration. Distances of the migration were used as signal readouts, which could be detected by naked eye. PDA-antiCD81 as exosome-capture vesicles were optimized (e.g., size, reaction ratio, and concentration) and the paper designs were investigated (e.g., diameter of sample reservoir and lamination layer) to enhance the solvent stop-flow effects. Finally, exosome screening on three cell culture samples (COLO1, MDA-MB-231, and HuR-KO1 subclone) was demonstrated. The method could linearly measure exosome concentrations in correlation with solvent migration distances in the range of 106–1010 particles/mL (R2 > 0.98) from the cell culture samples. The exosome concentration measurements by the developed device were independently assessed by nanoparticle tracking analysis. Results demonstrated no statistically significant difference (p > 0.05) by t-test. This low-cost and rapid device allows a portable platform for exosome quantification without the requirement of expensive equipment and expertise of operation. The developed device could potentially be useful for quantification of other biomarker-related extracellular vesicles. © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim |
|
dc.subject |
CD81 antigen |
|
dc.subject |
polyacetylene derivative |
|
dc.subject |
Article |
|
dc.subject |
cell aggregation |
|
dc.subject |
cell assay |
|
dc.subject |
cell clone |
|
dc.subject |
cell culture |
|
dc.subject |
cell vacuole |
|
dc.subject |
COLO1 cell line |
|
dc.subject |
concentration (parameter) |
|
dc.subject |
controlled study |
|
dc.subject |
equipment design |
|
dc.subject |
exosome |
|
dc.subject |
exosome capture vesicle |
|
dc.subject |
HuR-KO1 cell line |
|
dc.subject |
MDA-MB-231 cell line |
|
dc.subject |
process development |
|
dc.subject |
process optimization |
|
dc.subject |
stop flow paper based portable device |
|
dc.title |
Exosome aggregation mediated stop-flow paper-based portable device for rapid exosome quantification |
|
dc.type |
Article |
|
dc.rights.holder |
Scopus |
|
dc.identifier.bibliograpycitation |
Electrophoresis. Vol 41, (2020), p.311-318 |
|
dc.identifier.doi |
10.1002/elps.201900323 |
|