dc.contributor.author |
Wachiralurpan S. |
|
dc.contributor.author |
Chansiri K. |
|
dc.contributor.author |
Lieberzeit P.A. |
|
dc.date.accessioned |
2021-04-05T03:01:34Z |
|
dc.date.available |
2021-04-05T03:01:34Z |
|
dc.date.issued |
2020 |
|
dc.identifier.issn |
9254005 |
|
dc.identifier.other |
2-s2.0-85077926521 |
|
dc.identifier.uri |
https://ir.swu.ac.th/jspui/handle/123456789/11975 |
|
dc.identifier.uri |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85077926521&doi=10.1016%2fj.snb.2020.127678&partnerID=40&md5=eb54423301fb91b3cb13e1a2e531be23 |
|
dc.description.abstract |
Current methods for identifying Listeria monocytogenes are both time and labor intensive. However, it is highly desirable to detect it rapidly and reliably to prevent and/or identify contamination of foodstuff. Herein we propose a mass-sensitive sensor operating at elevated temperatures, 50−55 °C, for that purpose. Sensitive and selective detection relies on distinguishing genes of genomic extract of L. monocytogenes. A thiol-modified ssDNA probe designed for virulence phosphatidylcholine-phospholipase C (plcB) immobilized on the Quartz Crystal Microbalance (QCM) serves as the recognition element. This hybridizes with synthetic Loop-mediated isothermal amplification (LAMP) products of target DNA on the active surface sensor. Discernible detection limits of approximately 3 × 10−1 to 3 × 100 CFU mL-1 of L. monocytogenes DMST 17303 gDNA were achieved. The QCMDNA sensor showed high sensitivity and selectivity for L. monocytogenes (100 %) with negligible interference by DNA of other foodborne pathogens, such as Salmonella Paratyphi A (24 %), Salmonella Weltevreden (24 %), Salmonella Typhi (16 %), Shigella boydii (22 %), and Shigella flexneri (13 %). The temperature covered is in the range of 50–55 °C for immobilizing DNA probe and DNA target hybridization. Hybridization response times were within 10−30 min, demonstrated by saturation of the respective sensor responses. It turned out that sensitivity of the hybridization response increases up to two times by co-immobilizing the probe and L-cysteine. The latter acts as a spacer to increase probe-probe distance. This work demonstrates the potential of the QCM sensor technique at elevated temperatures as a sensor platform for further development of sensitive, specific and rapid detection of microbial DNA. © 2020 Elsevier B.V. |
|
dc.subject |
Amino acids |
|
dc.subject |
Diseases |
|
dc.subject |
DNA |
|
dc.subject |
Isotherms |
|
dc.subject |
Listeria |
|
dc.subject |
Probes |
|
dc.subject |
Quartz |
|
dc.subject |
Salmonella |
|
dc.subject |
Elevated temperature |
|
dc.subject |
Food-borne pathogens |
|
dc.subject |
Listeria monocytogenes |
|
dc.subject |
Loop mediated isothermal amplifications |
|
dc.subject |
Mass-sensitive sensor |
|
dc.subject |
Monocytogenes |
|
dc.subject |
plcBgene |
|
dc.subject |
Recognition element |
|
dc.subject |
Quartz crystal microbalances |
|
dc.title |
Direct detection of Listeria monocytogenes DNA amplification products with quartz crystal microbalances at elevated temperatures |
|
dc.type |
Article |
|
dc.rights.holder |
Scopus |
|
dc.identifier.bibliograpycitation |
Sensors and Actuators, B: Chemical. Vol 308, (2020) |
|
dc.identifier.doi |
10.1016/j.snb.2020.127678 |
|