Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/17399
Title: An automated fast-flow/delayed paper-based platform for the simultaneous electrochemical detection of hepatitis B virus and hepatitis C virus core antigen
Authors: Boonkaew S.
Yakoh A.
Chuaypen N.
Tangkijvanich P.
Rengpipat S.
Siangproh W.
Chailapakul O.
Keywords: Antigens
Channel flow
Chemical detection
Microfluidics
Paper
Viruses
ELectrochemical detection
Electrochemicals
Hepatitis B surface antigen
Hepatitis B virus
Hepatitis C virus
Hepatitis C virus core antigen
Microfluidic paper-based analytical device
Multi-step
Paper-based analytical devices
Point-of-care testing
Analytic equipment
biological marker
buffer
graphene
hepatitis B core antigen
hepatitis B surface antigen
oxidoreductase
potassium chloride
hepatitis B surface antigen
hepatitis C antigen
Article
blood sampling
chronoamperometry
cyclic voltammetry
electrochemical analysis
electrochemical detection
electron transport
hepatitis B
Hepatitis B virus
hepatitis C
Hepatitis C virus
human
impedance spectroscopy
microfluidics
point of care testing
genetic procedures
Hepacivirus
hepatitis B
Hepatitis B virus
Biosensing Techniques
Electrochemical Techniques
Hepacivirus
Hepatitis B
Hepatitis B Surface Antigens
Hepatitis B virus
Hepatitis C Antigens
Humans
Issue Date: 2021
Abstract: Electrochemical paper-based analytical devices (ePADs) are useful analytical devices that serve as point-of-care testing (POCT) devices for various clinical biomarkers in view of their simplicity, portability, and low-cost format. However, multistep reagent manipulation usually restricts the performance of the device for end users. Herein, we developed a sequential ePAD for sequential immunosensing fluid delivery by integrating dual flow behaviors (fast-flow/delayed) within a single paper platform for the simultaneous detection of hepatitis B surface antigen (HBsAg) and hepatitis C core antigen (HCVcAg). In the present work, a fast-flow channel was used for the automated washing of unbound antigens, while a delayed channel was created to store a redox reagent for further electrochemical analysis with a single buffer loading (the analysis time can be completed within 500 s). Hence, the undesirable complex procedure of multi-step reagent manipulation is scarcely needed by the user. The detection limit of the proposed ePAD was as low as 18.2 pg mL−1 for HBsAg and 1.19 pg mL−1 for HCVcAg. In addition, this proposed ePAD was also proven to be effective in real clinical sera from patients to verify its biological applicability. The ePAD sensor shows high promise as an easy-to-use, portable, and extendable sensor for other multiplex biological assays. © 2021 Elsevier B.V.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85112754391&doi=10.1016%2fj.bios.2021.113543&partnerID=40&md5=34deba761eacc8172587bb051c8acb34
http://ir.swu.ac.th/jspui/handle/123456789/17399
ISSN: 9565663
Appears in Collections:Scopus 1983-2021

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