Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/15399
Title: A specific DNA probe which identifies Babesia bovis in whole blood
Authors: Petchpoo W.
Tan-ariya P.
Boonsaeng V.
Brockelman C.R.
Wilairat P.
Panyim S.
Keywords: animal experiment
article
babesiosis
cattle
controlled study
dna probe
immunoblotting
nonhuman
Animal
Babesia bovis
Babesiosis
Blotting, Southern
Cattle
Cattle Diseases
Cloning, Molecular
DNA Probes
DNA, Protozoan
Erythrocytes
Gene Library
Nucleic Acid Hybridization
Plasmids
Restriction Mapping
Sensitivity and Specificity
Support, Non-U.S. Gov't
Transformation, Genetic
Acari
Anaplasma marginale
Animalia
Babesia
Babesia bigemina
Babesia bovis
Boophilus bovis
Boophilus microplus
Bos taurus
Escherichia coli
Plasmodium falciparum
Trypanosoma
Trypanosoma evansi
Issue Date: 1992
Abstract: A genomic library of Babesia bovis DNA from the Mexican strain M was constructed in plasmid pUN121 and cloned in Escherichia coli. Several recombinants which hybridized strongly to radioactively labeled B. bovis genomic DNA in an situ screening were selected and further analyzed for those which specifically hybridized to B. bovis DNA. It was found that pMU-B1 had the highest sensitivity, detecting 25 pg of purified B. bovis DNA, and 300 parasites in 10 μl of whole infected blood, or 0.00025% parasiteemia. pMU-B1 contained a 6.0 kb B. bovis DNA insert which did not cross-hybridize to Babesia bigemina, Trypanosoma evansi, Plasmodium falciparum, Anaplasma marginale, Boophilus microplus and cow DNA. In the Southern blot analysis of genomic DNA, pMU-B1 could differentiate between two B. bovis geographic isolates, Mexican strain M and Thai isolate TS4. Thus, the pMU-B1 probe will be useful in the diagnosis of Babesia infection in cattle and ticks, and in the differentiation of B. bovis strains. © 1992.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026636008&doi=10.1016%2f0304-4017%2892%2990061-D&partnerID=40&md5=3c381434c2b679e8f500ec8c6e45b827
http://ir.swu.ac.th/jspui/handle/123456789/15399
ISSN: 3044017
Appears in Collections:Scopus 1983-2021

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