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Title: Yellow head virus from Thailand and gill-associated virus from Australia are closely related but distinct prawn viruses
Authors: Cowley J.A.
Dimmock C.M.
Wongteerasupaya C.
Boonsaeng V.
Panyim S.
Walker P.J.
Keywords: amino acid sequence
gill associated virus
marine environment
nucleic acid probe
primer DNA
reverse transcription polymerase chain reaction
sequence analysis
virus detection
virus genome
yellow head virus
Gill-associated virus
Penaeus monodon
Thailand virus
Yellow head virus
Issue Date: 1999
Abstract: Corresponding genomic regions of isolates of yellow head virus (YHV) from Thailand and gill-associated virus (GAV) from Australia were compared by RT-PCR and sequence analysis. PCR primers designed from sequences in the GAV ORF1b polyprotein gene amplified the corresponding 577 nucleotide region of the YHV genome. Comparison of the amplified region indicated 85.1% nucleotide and 95.8% amino acid sequence identity. YHV PCR primers designed to amplify a 135 nucleotide product previously described as a YHV diagnostic probe failed to amplify the corresponding product from GAV RNA. However, the cognate GAV sequence for this and another recently reported YHV sequence were located in an upstream region of the ORF1b gene. A comparison of these sequences indicated identities of 83.0 and 80.9% at the nucleotide level and 86.7 and 86.5% at the amino acid level, respectively. The data indicate that GAV and YHV are closely related but distinct viruses for which differential diagnostic probes can be applied.
ISSN: 1775103
Appears in Collections:Scopus 1983-2021

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