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Title: Molecular phylogenetic studies on Theileria parasites based on small subunit ribosomal RNA gene sequences
Authors: Chansiri K.
Kawazu S.-I.
Kamio T.
Terada Y.
Fujisaki K.
Philippe H.
Sarataphan N.
Keywords: ribosome DNA
animal experiment
nucleotide sequence
parasite identification
polymerase chain reaction
theileria parva
Asia, Southeastern
Base Sequence
DNA, Protozoan
Molecular Sequence Data
Polymerase Chain Reaction
RNA, Ribosomal
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Martes zibellina
Theileria annulata
Theileria buffeli
Theileria parva
Theileria sergenti
Theileria taurotragi
unclassified Theileria
Issue Date: 1999
Abstract: Classification of Theileria parasites of south-east Asian countries is still ambiguous due to the lack of basic studies, especially their molecular genetic information. In this study, we included 6 known species and 14 unclassified Theileria parasite isolates: Theileria annulata, Theileria parva, Theileria taurotragi, Theileria sergenti, Theileria buffeli, Theileria types Sable, Theileria types A, B, B1, B2, C, D, E, F, G, G1, Theileria type Medan (Indonesia), Theileria type Ipoh (Malaysia) and Theileria type Thong Song (Thailand). Small subunit ribosomal RNA (srRNA) nucleotide sequence data were collected by PCR, cloning and dideoxy sequencing. The srRNA nucleotide sequences were aligned and analyzed by distance methods, maximum parsimony algorithms and maximum likelihood methods to construct phylogenetic trees. Bootstrap analysis was used to test the strength of the different phylogenetic reconstructions. The data indicated that all of the tree-building methods gave very similar results. This study identified two groups of Theileria, the pathogenic and benign groups, which are strongly supported by bootstrap analysis. The analysis also indicated that three subgroups (A, B and C) were generated within the benign Theileria group whereas the classification of Theileria type D and Thong Song is questionable. However, more basic information such as life cycle differences, vectors, modes of transmission, virulent and genetic/sexual compatability is essential for clearer taxonomic definition of the benign Theileria parasites. Copyright (C) 1999 Elsevier Science B.V.
ISSN: 3044017
Appears in Collections:SCOPUS 1983-2021

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