Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/15236
Title: PCR-ELISA for diagnosis of Trypanosoma evansi in animals and vector
Authors: Chansiri K.
Khuchareontaworn S.
Nopporn S.
Keywords: DNA
protozoal DNA
analytic method
Anaplasma
Anaplasma marginale
article
Babesia
Babesia bigemina
Babesia bovis
buffalo
cattle
controlled study
cross reaction
deer
diagnostic accuracy
diagnostic value
enzyme linked immunosorbent assay
mosquito
mouse
nonhuman
polymerase chain reaction
priority journal
sensitivity and specificity
swine
Thailand
Theileria
Trypanosoma evansi
animal
animal disease
comparative study
disease carrier
enzyme linked immunosorbent assay
evaluation
genetics
methodology
parasitology
polymerase chain reaction
standard
Trypanosoma
trypanosomiasis
Anaplasma
Anaplasma marginale
Animalia
Babesia
Theileria
Theileria sp.
Trypanosoma
Trypanosoma evansi
Animals
Buffaloes
Cattle
Cross Reactions
Culicidae
Deer
DNA, Protozoan
Enzyme-Linked Immunosorbent Assay
Insect Vectors
Mice
Polymerase Chain Reaction
Sensitivity and Specificity
Trypanosoma
Trypanosomiasis
Issue Date: 2002
Abstract: A highly sensitive and specific polymerase chain reaction (PCR) based assay for the detection of Trypanosoma evansi present in the blood of different animals and vector was developed. A simple lysis method was used to remove of the red blood cells to facilitate direct input of samples into the PCR reactions. The primer set was designed and synthesized to amplify a single band of 257 bp PCR product that was subsequently examined by enzyme-linked immunosorbent assay (ELISA). The sensitivity limit of PCR-ELISA was 0.01 pg that was corresponded to 1 parasite/ml of blood. No cross-reactivity of the assay was observed against Babesia boris, B. bigemina, Anaplasma marginale, Theileria sp. and host DNA. The PCR-ELISA was shown to detect 33 samples of T. evansi infected blood of animals and 10 mosquitoes from different geographical area in Thailand. The results were corresponded to those of the PCR and mouse inoculation. This implies that the technique of PCR-ELISA is not only beneficial for diagnosis of the parasite but also useful for epidemiological study and designing rational trypanosomiasis control program. © 2002 Elsevier Science Ltd. All rights reserved.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036599194&doi=10.1006%2fmcpr.2002.0412&partnerID=40&md5=16244021d9b25f79a513e1a66c9c4cb0
http://ir.swu.ac.th/jspui/handle/123456789/15236
ISSN: 8908508
Appears in Collections:SCOPUS 1983-2021

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