Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/15233
Title: PCR based method for identification of zoonostic Brugia malayi microfilariae in domestic cats
Authors: Chansiri K.
Tejangkura T.
Kwaosak P.
Sarataphan N.
Phantana S.
Sukhumsirichart W.
Keywords: acid phosphatase
analytic method
animal experiment
animal model
article
blood sampling
Brugia malayi
cat
controlled study
exon
infection control
Microfilaria
nonhuman
periodicity
polymerase chain reaction
priority journal
restriction fragment length polymorphism
risk assessment
Thailand
zoonosis
animal
animal disease
Brugia malayi
cat disease
classification
disease carrier
endemic disease
filariasis
genetics
human
isolation and purification
methodology
Microfilaria
parasitemia
parasitology
Thailand
zoonosis
Animalia
Brugia
Brugia malayi
Felidae
Felis catus
Animals
Brugia malayi
Cat Diseases
Cats
Disease Reservoirs
Endemic Diseases
Filariasis
Humans
Microfilaria
Parasitemia
Polymerase Chain Reaction
Thailand
Zoonoses
Issue Date: 2002
Abstract: The survey of 326 human blood samples in the endemic area of Surat Thani and Narathiwat, the provinces in the south of Thailand, revealed that 5 of them were infected with Brugia malayi. Similarly, 53 feline blood samples were also investigated and found that 15 of the domestic cats were also infected with B. malayi. Upon the examination of human and feline blood specimens, a pair of human and domestic cat stayed in the same house and region. The periodicities of human B. malayi and feline B. malayi were similar as well as the results of Giemsa and acid phosphatase stained blood films of microfilaria positive cases. Likewise, the PCR-RFLP profile of Hha I repeat genes and PCR amplification of Trans-Spliced Leader Exon I (SLX) demonstrated that 15 samples the feline B. malayi were the same as those of human B. malayi. The data indicated that domestic cat plays an important role as the animal reservoir for B. malayi in the endemic areas of Thailand. © 2002 Elsevier Science Ltd.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036018473&doi=10.1006%2fmcpr.2001.0402&partnerID=40&md5=29911bcc49e9df485f4b7a3b95d855c8
http://ir.swu.ac.th/jspui/handle/123456789/15233
ISSN: 8908508
Appears in Collections:SCOPUS 1983-2021

Files in This Item:
There are no files associated with this item.


Items in SWU repository are protected by copyright, with all rights reserved, unless otherwise indicated.