Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/15208
Title: UTP-dependent inhibition of Na+ absorption requires activation of PKC in endometrial epithelial cells
Authors: Palmer-Densmore M.
Deachapunya C.
Kannan M.
O'Grady S.M.
Keywords: 12 (2 cyanoethyl) 6,7,12,13 tetrahydro 13 methyl 5 oxoindolo[2,3 a]pyrrolo[3,4 c]carbazole
2 [1 (3 dimethylaminopropyl) 3 indolyl] 3 (3 indolyl)maleimide
2 [1 (3 dimethylaminopropyl) 5 methoxy 1h indol 3 yl] 3 (1h indol 3 yl)maleimide
benzamil
calcium ion
chloride ion
ethylene glycol 1,2 bis(2 aminophenyl) ether n,n,n',n' tetraacetic acid
phorbol 13 acetate 12 myristate
protein kinase C
protein kinase C inhibitor
sodium channel
sodium ion
uridine triphosphate
carrier protein
protein kinase C
protein kinase modulator
signal peptide
sodium
uridine triphosphate
animal cell
article
calcium activated sodium channel
cell membrane permeability
cell secretion
controlled study
endometrium
enzyme activation
epithelium cell
nonhuman
sodium absorption
sodium conductance
swine
absorption
animal
cytology
drug antagonism
drug effect
enzyme activation
enzymology
female
metabolism
physiology
Absorption
Animals
Carrier Proteins
Endometrium
Enzyme Activation
Epithelial Cells
Female
Intracellular Signaling Peptides and Proteins
Protein Kinase C
Sodium
Swine
Uridine Triphosphate
Issue Date: 2002
Abstract: The objective of this study was to investigate the mechanism of uridine 5′-triphosphate (UTP)-dependent inhibition of Na+ absorption in porcine endometrial epithelial cells. Acute stimulation with UTP (5 μM) produced inhibition of sodium absorption and stimulation of chloride secretion. Experiments using basolateral membrane-permeabilized cell monolayers demonstrated a reduction in benzamil-sensitive Na+ conductance in the apical membrane after UTP stimulation. The UTP-dependent inhibition of sodium transport could be mimicked by PMA (1 μM). Several PKC inhibitors, including GF109203X and Gö6983 (both nonselective PKC inhibitors) and rottlerin (a PKCδ selective inhibitor), were shown to prevent the UTP-dependent decrease in benzamil-sensitive current. The PKCα-selective inhibitors, Gö6976 and PKC inhibitor 20-28, produced a partial inhibition of the UTP effect on benzamil-sensitive Isc. Inhibition of the benzamil-sensitive Isc by UTP was observed in the presence of BAPTA-AM (50 μM), confirming that activation of PKCs, and not increases in [Ca2+]i, were directly responsible for the inhibition of apical Na+ channels and transepithelial Na+ absorption.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-1842856100&doi=10.1085%2fjgp.20028608&partnerID=40&md5=00237eae14c44670a678c655d3165e36
http://ir.swu.ac.th/jspui/handle/123456789/15208
ISSN: 221295
Appears in Collections:Scopus 1983-2021

Files in This Item:
There are no files associated with this item.


Items in SWU repository are protected by copyright, with all rights reserved, unless otherwise indicated.