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https://ir.swu.ac.th/jspui/handle/123456789/15127| Title: | Detection of lymphatic Wuchereria bancrofti in carriers and long-term storage blood samples using semi-nested PCR |
| Authors: | Kanjanavas P. Tan-Ariya P. Khawsak P. Pakpitcharoen A. Phantana S. Chansiri K. |
| Keywords: | DNA selfish dna unclassified drug accuracy article blood analysis blood storage Brugia malayi controlled study DNA determination DNA sequence gene amplification nonhuman parasite detection parasite examination Plasmodium falciparum Plasmodium vivax polymerase chain reaction priority journal sensitivity and specificity Wuchereria bancrofti Brugia malayi Plasmodium falciparum Plasmodium vivax Wuchereria bancrofti |
| Issue Date: | 2005 |
| Abstract: | The semi-nested PCR was conducted for detection of Wuchereria bancrofti in patients' blood. The primers were designed based on the repetitive DNA sequences of the parasite. The results demonstrated that the semi-nested PCR could detect as little as 0.001 fg of parasite DNA. In addition, the primers showed no PCR amplification from human and other hemoparasites such as Brugia malayi, Plasmodium falciparum and P. vivax DNAs. This technique was used for detection of 18 W. bancrofti infected blood samples with a long-term storage, the data revealed that all samples were positive. The results obtained from this study clearly indicated that the semi-nested PCR is specific, sensitive, and suitable for detection of the disease carriers. © 2004 Elsevier Ltd. All rights reserved. |
| URI: | https://ir.swu.ac.th/jspui/handle/123456789/15127 https://www.scopus.com/inward/record.uri?eid=2-s2.0-15944415828&doi=10.1016%2fj.mcp.2004.11.003&partnerID=40&md5=19f7ad9eb19c96dc3ee332082614c866 |
| ISSN: | 8908508 |
| Appears in Collections: | Scopus 1983-2021 |
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