Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/15084
Title: Barakol extracted from cassia siamea stimulates chloride secretion in rat colon
Authors: Deachapunya C.
Poonyachoti S.
Thongsaard W.
Krishnamra N.
Keywords: amiloride
atropine
barakol
bumetanide
chloride
efenamic acid
glibenclamide
hexamethonium
indometacin
plant extract
prostaglandin synthase
Ringer solution
tetrodotoxin
unclassified drug
animal tissue
anion transport
article
ascending colon
chloride transport
colon mucosa
concentration response
descending colon
Eassia simea
intestine secretion
male
nonhuman
priority journal
rat
Senna
short circuit current
Animals
Anti-Anxiety Agents
Benzopyrans
Bicarbonates
Calcium Channel Blockers
Cassia
Chlorides
Colon
Cyclooxygenase Inhibitors
Electrophysiology
Indomethacin
Intestinal Mucosa
Male
Phenalenes
Rats
Rats, Wistar
Sodium-Potassium-Chloride Symporters
Stimulation, Chemical
Issue Date: 2005
Abstract: Barakol is a purified extract of Cassia siamea, a plant that has been used as a laxative in traditional medicine. In this study, the effect of barakol on anion transport across the rat colon epithelium was investigated. Colonic epithelium was mounted in Ussing chambers and bathed with Ringer's solution. Addition of 1 mM barakol to the basolateral solution produced a slow increase in short-circuit current (Isc) in proximal colon and distal colon by 24.5 ± 2.2 and 24.2 ± 1.4 μA/cm2, respectively. Barakol increased Isc in a concentration-dependent manner with an EC50 value of 0.4 mM. The barakol-stimulated increase in Isc was inhibited by subsequent treatment with 500 μM diphenylamine-2-carboxylic acid or 400 μM glibenclamide added to the apical solution and 200 μM bumetanide added to the basolateral solution. Pretreatment of the tissues with 200 μM bumetanide, but not 10 μM amiloride, completely abolished the barakol-increased Isc. Ion substitution experiments showed an inhibition of barakol-stimulated Isc in chloride-free solution but not in bicarbonate-free solution. In addition, pretreatment of tissues with 10 μM tetrodotoxin or 10 μM indomethacin, but not 1 μM atropine or 10 μM hexamethonium, partially inhibited the Isc response by barakol. The present results demonstrated the stimulatory effect of barakol on the bumetanide-sensitive chloride secretion in rat colon. The effect of barakol was partly mediated by the stimulation of submucosal nerves and through the release of cyclooxygenase metabolites. These findings thus provide an explanation for the underlying mechanism of barakol as a secretagogue in mammalian colon. Copyright © 2005 by The American Society for Pharmacology and Experimental Therapeutics.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-22944487650&doi=10.1124%2fjpet.105.084210&partnerID=40&md5=ee7670712d1ae43839848193861ac34e
http://ir.swu.ac.th/jspui/handle/123456789/15084
ISSN: 223565
Appears in Collections:Scopus 1983-2021

Files in This Item:
There are no files associated with this item.


Items in SWU repository are protected by copyright, with all rights reserved, unless otherwise indicated.