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Title: Development of a polyclonal antibody specific to VP19 envelope protein of white spot syndrome virus (WSSV) using a recombinant protein preparation
Authors: Chaivisuthangkura P.
Phattanapaijitkul P.
Thammapalerd N.
Rukpratanporn S.
Longyant S.
Sithigorngul W.
Sithigorngul P.
Keywords: envelope protein
maltose binding protein
monoclonal antibody
polyclonal antibody
protein vp 19
recombinant protein
structural protein
unclassified drug
polyacrylamide gel electrophoresis
polymerase chain reaction
priority journal
Western blotting
White spot syndrome virus
Antibodies, Monoclonal
Antibodies, Viral
Blotting, Western
DNA, Viral
Electrophoresis, Polyacrylamide Gel
Escherichia coli
Genes, Viral
Recombinant Proteins
Viral Envelope Proteins
White spot syndrome virus 1
Penaeus monodon
Shrimp white spot syndrome virus
Issue Date: 2006
Abstract: The VP19 gene encoding a structural envelope protein of white spot syndrome virus was cloned into an expression vector and introduced into E. coli. The objective was to produce a recombinant VP19 structural protein. After induction, the recombinant VP19 protein (rVP19) was produced, purified by SDS-PAGE and used for immunization of Swiss mice for polyclonal antibody production. The mouse anti rVP19 antiserum had specific immunoreactivity to the viral antigen in WSSV infected Penaeus monodon as verified by immunohistochemistry and Western blot. The production of monoclonal antibodies against this rVP19 may be useful in order to combine with anti-VP28 monoclonal antibodies for enhancing the sensitivity of various WSSV serological assays. © 2006 Elsevier B.V. All rights reserved.
ISSN: 1660934
Appears in Collections:SCOPUS 1983-2021

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