Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/14790
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dc.contributor.authorChaivisuthangkura P.
dc.contributor.authorSrisuk C.
dc.contributor.authorRukpratanporn S.
dc.contributor.authorLongyant S.
dc.contributor.authorSridulyakul P.
dc.contributor.authorSithigorngul P.
dc.date.accessioned2021-04-05T04:31:56Z-
dc.date.available2021-04-05T04:31:56Z-
dc.date.issued2009
dc.identifier.issn1660934
dc.identifier.other2-s2.0-70349734867
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-70349734867&doi=10.1016%2fj.jviromet.2009.08.005&partnerID=40&md5=64e9584e36652e7239aef5081252f320
dc.identifier.urihttp://ir.swu.ac.th/jspui/handle/123456789/14790-
dc.description.abstractLoop-mediated isothermal amplification (LAMP) is a novel, sensitive and rapid method for amplification of nucleic acids under isothermal conditions. In this report, a LAMP method was developed for detection of Penaeus monodon nucleopolyhedrovirus (PemoNPV), known previously as monodon baculovirus (MBV), using a set of six primers designed to specifically recognize the PemoNPV polyhedrin gene. The optimized time and temperature conditions for the LAMP assay were 60 min at 63 °C. The sensitivity of LAMP for PemoNPV detection was approximately 50 viral copies ng-1 genomic DNA (equivalent to 150 viral copies per reaction). Using a DNA template extracted from PemoNPV-infected shrimp by a viral nucleic acid kit, the detection limit of LAMP was 0.7 fg while that of nested PCR was 70 fg; therefore, the LAMP assay was 100 times more sensitive than nested PCR. The LAMP method did not amplify a product using nucleic acid extracted from shrimp infected with other viruses including yellow head virus (YHV), Taura syndrome virus (TSV), white spot syndrome virus (WSSV), Penaeus stylirostris densovirus (PstDNV) known previously as infectious hypodermal and hematopoietic necrosis virus (IHHNV), and Penaeus monodon densovirus (PmDNV) known previously as hepatopancreatic parvovirus (HPV). © 2009 Elsevier B.V. All rights reserved.
dc.subjectgenomic DNA
dc.subjectpolyhedrin
dc.subjectanalytical equipment
dc.subjectarticle
dc.subjectDensovirus
dc.subjectDNA extraction
dc.subjectInfectious hematopoietic necrosis virus
dc.subjectloop mediated isothermal amplification
dc.subjectnonhuman
dc.subjectnucleic acid amplification
dc.subjectnucleotide sequence
dc.subjectParvovirus
dc.subjectPenaeus monodon nucleopolyhedrovirus
dc.subjectPenaeus stylirostris densovirus
dc.subjectplasmid
dc.subjectPolyhedrosis virus
dc.subjectpolymerase chain reaction
dc.subjectpriority journal
dc.subjectReovirus
dc.subjectsensitivity analysis
dc.subjectshrimp
dc.subjectTaura syndrome virus
dc.subjecttemperature
dc.subjectvirus detection
dc.subjectvirus gene
dc.subjectvirus infection
dc.subjectWhite spot syndrome virus
dc.subjectyellow head virus
dc.subjectAnimals
dc.subjectDNA Primers
dc.subjectNucleic Acid Amplification Techniques
dc.subjectNucleopolyhedrovirus
dc.subjectPenaeidae
dc.subjectPolymerase Chain Reaction
dc.subjectSensitivity and Specificity
dc.subjectSpecies Specificity
dc.subjectTime Factors
dc.subjectViral Structural Proteins
dc.subjectDecapoda (Crustacea)
dc.subjectDensovirus
dc.subjectHepatopancreatic parvovirus of penaeid shrimp
dc.subjectInfectious hypodermal and hematopoietic necrosis virus
dc.subjectLitopenaeus stylirostris
dc.subjectMonodon baculovirus
dc.subjectNucleopolyhedrovirus
dc.subjectPenaeus monodon
dc.subjectShrimp white spot syndrome virus
dc.subjectTaura syndrome virus
dc.subjectYellow head virus
dc.titleRapid and sensitive detection of Penaeus monodon nucleopolyhedrovirus by loop-mediated isothermal amplification
dc.typeArticle
dc.rights.holderScopus
dc.identifier.bibliograpycitationJournal of Virological Methods. Vol 162, (2009), p.188-193
dc.identifier.doi10.1016/j.jviromet.2009.08.005
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