Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/14785
Title: Efficient delivery of antisense oligodeoxyribonucleotide G3139 by human serum albumin-coated liposomes
Authors: Weecharangsan W.
Yu B.
Zheng Y.
Liu S.
Pang J.X.
Lee L.J.
Marcucci G.
Lee R.J.
Keywords: alpha tocopherol
dimethyldioctadecyl ammonium bromide
human serum albumin
liposome
oblimersen
phosphatidylcholine
protein bcl 2
unclassified drug
alpha helix
article
carcinoma cell
complex formation
controlled study
down regulation
drug formulation
genetic transfection
human
human cell
mouth carcinoma
priority journal
Blotting, Western
Cell Line, Tumor
Circular Dichroism
Electrophoresis, Agar Gel
Humans
Liposomes
Proto-Oncogene Proteins c-bcl-2
Reverse Transcriptase Polymerase Chain Reaction
Serum Albumin
Thionucleotides
Issue Date: 2009
Abstract: Human serum albumin (HSA)-coated liposomal formulations were synthesized and evaluated for the delivery of antisense oligodeoxyribonucleotide (ODN) G3139 in KB human oral carcinoma cells. Liposomes composed of dimethyldioctadecyl ammonium bromide/egg phosphatidylcholine/α-tocopheryl polyethylene glycol 1000 succinate (58:40:2 molar ratio) complexed with G3139 and coated with HSA were investigated for Bcl-2 downregulating activity. Cellular uptake of HSA-coated liposome-ODN complexes was more efficient than the uncoated liposome-ODN complexes. Treatment of the cells with HSA-coated liposome-ODN complexes resulted in efficient Bcl-2 mRNA downregulation that was approximately 3-fold greater than with uncoated liposomes (p < 0.05) and 6-fold greater than with free ODN. The transfection efficiency of liposome-ODN complexes coated with HSA was dependent on the concentration of HSA used and on the contents of α-helix and β-strand in HSA. HSA-coated liposomes are effective delivery vehicles for antisense ODN. © 2009 American Chemical Society.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-72049109189&doi=10.1021%2fmp900150g&partnerID=40&md5=61f2c9acba8df78794d86ee7603ae159
http://ir.swu.ac.th/jspui/handle/123456789/14785
ISSN: 15438384
Appears in Collections:SCOPUS 1983-2021

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