Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/14706
Title: Effect of human β-globin bacterial artificial chromosome transgenesis on embryo cryopreservation in mouse models
Authors: Boonkusol D.
Dinnyes A.
Faisaikarm T.
Sangsuwan P.
Pratipnatalang N.
Sa-Ardrit M.
Saikhun K.
Svasti S.
Vadolas J.
Winichagoon P.
Fucharoen S.
Kitiyanant Y.
Keywords: beta globin
ethylene glycol
animal experiment
animal tissue
article
bacterial artificial chromosome
blastocyst
breeding line
controlled study
cryopreservation
embryo
embryo cryopreservation
embryo development
embryo transfer
female
gene expression
heterozygote
in vivo study
male
mouse
mouse embryo
nidation
nonhuman
survival rate
transgene
transgenics
vitrification
wild type
Animals
beta-Globins
Chromosomes, Artificial, Bacterial
Cryopreservation
Embryo Culture Techniques
Embryo Transfer
Embryo, Mammalian
Female
Gene Expression
Gene Transfer Techniques
Heterozygote
Humans
Male
Mice
Mice, Inbred C57BL
Mice, Inbred ICR
Mice, Knockout
Mice, Transgenic
Thalassemia
Animalia
Bacteria (microorganisms)
Issue Date: 2010
Abstract: The purpose of the present study was to investigate the efficiency of embryo cryopreservation for four transgenic (TG) thalassaemic mouse strains, which is a key element of the ongoing gene banking efforts for these highvalue animals. Heterozygous TG embryos were produced by breeding four lines of TG males to wild-type (WT) females (C57BL/6J). Intact two-cell embryos were cryopreserved by vitrification in straws using 35% ethylene glycol. Survival rates of cryopreserved embryos ranged between 91.1% (102/112) and 93.6% (176/188) without significant differences between the lines. In contrast, the paternal line had a significant effect on the development of these embryos to the blastocyst stage, which ranged from 50.6% (92/182) to 77.5% (79/102). This effect was also noted following embryo transfers, with implantation rates varying from 17.3% (19/110) to 78.1% (35/45). The results demonstrate that the in vivo developmental potential is significantly influenced byTG line and reveal a specific line effect on cryosurvival. All bacterial artificial chromosome transgenic fetuses developed from vitrified-warmed embryos showed expression of the human β-globin transgene. In conclusion, the present study shows a strongTG line effect on developmental competence following cryopreservation and the vitrification method was successful to bank the human β-globin TG-expressing mouse strains. © 2010 CSIRO.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-77951784898&doi=10.1071%2fRD09128&partnerID=40&md5=aa76013bb9105ffd5bf468b8b3828a30
http://ir.swu.ac.th/jspui/handle/123456789/14706
ISSN: 10313613
Appears in Collections:Scopus 1983-2021

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