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Title: Vanillin enhances TRAIL-induced apoptosis in cancer cells through inhibition of NF-κB activation
Authors: Lirdprapamongkol K.
Sakurai H.
Suzuki S.
Koizumi K.
Prangsaengtong O.
Viriyaroj A.
Ruchirawat S.
Svasti J.
Saiki I.
Keywords: caspase 3
immunoglobulin enhancer binding protein
mitogen activated protein kinase
nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase 1
phosphatidylinositol 3 kinase
synaptotagmin I
tumor necrosis factor related apoptosis inducing ligand
cell count
cell viability
drug mechanism
enzyme activation
HeLa cell
human cell
protein cleavage
protein phosphorylation
uterine cervix cancer
Cell Death
Cell Survival
Genes, Reporter
Hela Cells
NF-kappa B
TNF-Related Apoptosis-Inducing Ligand
Uterine Cervical Neoplasms
Issue Date: 2010
Abstract: Background: Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a promising anticancer agent which selectively kills cancer cells with little effect on normal cells. However, TRAIL resistance is widely found in cancer cells. We have previously reported antimetatstatic and antiangiogenic effects of vanillin, a flavoring agent from vanilla. Here we have evaluated the sensitizing effect of vanillin on a TRAIL-resistant human cervical cancer cell line, HeLa. Materials and Methods: Cell viability after treatments was determined by the WST-1 cell counting kit. Apoptosis was demonstrated by detection of caspase-3 activation and cleavage of poly (ADP-ribose) polymerase using immunoblot analysis. Effect of treatments on TRAIL signaling pathway and nuclear factor κB (FN-κB) activation was studied using immunoblot analysis and luciferase reporter assay. Results: Pretreatment of HeLa cells with vanillin enhanced TRAIL-induced cell death through the apoptosis pathway. Vanillin pretreatment inhibited TRAIL-induced phosphorylation of p65 and transcriptional activity of NF-κB. Conclusion: Vanillin sensitizes HeLa cells to TRAIL-induced apoptosis by inhibiting NF-κB activation.
ISSN: 0258851X
Appears in Collections:Scopus 1983-2021

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