Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/14630
Title: Bioproduction and anticancer activity of biosurfactant produced by the dematiaceous fungus Exophiala dermatitidis SK80
Authors: Chiewpattanakul P.
Phonnok S.
Durand A.
Marie E.
Thanomsub B.W.
Keywords: ammonium nitrate
biosurfactant
cell DNA
glycerol oleate
palm oil
animal cell
antineoplastic activity
article
catalysis
cell membrane
cell strain U937
cell structure
concentration response
controlled study
DNA fragmentation
DNA structure
drug cytotoxicity
drug purification
drug structure
drug synthesis
Exophiala
exophiala dermatitidis
fungal contamination
fungal morphology
fungal strain
fungus culture
fungus identification
HeLa cell
human
human cell
incubation time
nonhuman
pH
Antibiotics, Antineoplastic
Antineoplastic Agents
Cell Line, Tumor
Chromatography, Liquid
Culture Media
Exophiala
Humans
Magnetic Resonance Spectroscopy
Molecular Structure
Plant Oils
Silica Gel
Soil Microbiology
Surface-Active Agents
Exophiala
Exophiala dermatitidis
Fungi
Issue Date: 2010
Abstract: A new biosurfactant producer was isolated from palm-oilcontaminated soil and later identified through morphology and DNA sequencing as the yeast-like fungus Exophiala dermatitidis. Biosurfactant production was catalyzed by vegetable oil, supplemented with a basal medium. The culture conditions that provided the biosurfactant with the highest surface activity were found to be 5% palm oil with 0.08% NH4NO3, at a pH of 5.3, with shaking at 200 rpm, and a temperature of 30°C for a 14-day period of incubation. The biosurfactant was purified, in accordance with surfactant properties, by solvent fractionation using silica gel column chromatography. The chemical structure of the strongest surface-active compound was elucidated through the use of NMR and mass spectroscopy, and noted to be monoolein, which then went on to demonstrate antiproliferative activity against cervical cancer (HeLa) and leukemia (U937) cell lines in a dose-dependent manner. Interestingly, no cytotoxicity was observed with normal cells even when high concentrations were used. Cell and DNA morphological changes, in both cancer cell lines, were observed to be cell shrinkage, membrane blebbling, and DNA fragmentation.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-78650849958&doi=10.4014%2fjmb.1007.07052&partnerID=40&md5=6f4ece92135af6d8b8d01db1ef83d21e
http://ir.swu.ac.th/jspui/handle/123456789/14630
ISSN: 10177825
Appears in Collections:Scopus 1983-2021

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