Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/14516
Title: Photocleavage of avidin by a new pyrenyl probe
Authors: Malaikaew P.
Svasti J.
Kumar C.V.
Buranaprapuk A.
Keywords: avidin
biotin
peptide fragment
amino terminal sequence
article
binding site
chromatophore
fluorescence spectroscopy
irradiation
molecular library
photochemistry
photodynamics
priority journal
protein binding
protein structure
protein targeting
Absorption
Avidin
Binding Sites
Biotin
Chlorides
Cobalt
Electrophoresis, Polyacrylamide Gel
Fluorescent Dyes
Light
Peptide Fragments
Photolysis
Pyrenes
Spectrometry, Fluorescence
Threonine
Valine
Issue Date: 2011
Abstract: In this study, a new small-molecule-based reagent was designed to recognize and bind to specific site in protein. A new pyrenyl probe, d-biotinyl-1(1- pyrene)methylamide (Py-biotin) was designed and synthesized by coupling of d-biotin to 1(1-pyrene)methylamine hydrochloride. Binding studies and site-specific photocleavage of avidin by Py-biotin were demonstrated. Binding of Py-biotin to avidin was studied using absorbance and fluorescence spectroscopic techniques. Red shifts of the absorption peak positions of the pyrenyl chromophore followed by hyperchromism were observed upon binding to avidin. The photocleavage of avidin was achieved when a mixture of the protein, Py-biotin, and an electron acceptor, cobalt(III) hexammine trichloride (CoHA), was irradiated at 342 nm. No reaction occurred in the absence of the probe, CoHA, or light. N-terminal sequencing of the peptide fragments indicated a cleavage site of avidin between Thr 77 and Val 78. The high specificity of photocleavage may be valuable in targeting specific sites of proteins with small molecules. © 2011 Elsevier B.V. All rights reserved.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-79955937531&doi=10.1016%2fj.jphotobiol.2011.04.003&partnerID=40&md5=20f18b103cc5d78e03a5534cafd623f5
http://ir.swu.ac.th/jspui/handle/123456789/14516
ISSN: 10111344
Appears in Collections:SCOPUS 1983-2021

Files in This Item:
There are no files associated with this item.


Items in SWU repository are protected by copyright, with all rights reserved, unless otherwise indicated.