Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/14459
Title: Differentiation among the Vibrio cholerae serotypes O1, O139, O141 and non-O1, non-O139, non-O141 using specific monoclonal antibodies with dot blotting
Authors: Pengsuk C.
Longyant S.
Rukpratanporn S.
Chaivisuthangkura P.
Sridulyakul P.
Sithigorngul P.
Keywords: monoclonal antibody
peptone
article
bacterium culture
bacterium isolate
bacterium isolation
blotting
colony forming unit
controlled study
cross reaction
dot blotting
Gram negative bacterium
immunization
nonhuman
polymerase chain reaction
priority journal
serotype
Vibrio cholerae
Vibrio mimicus
Animals
Antibodies, Bacterial
Antibodies, Monoclonal
Antigens, Bacterial
Cholera
Feces
Female
Food Microbiology
Humans
Immunoblotting
Mice
Palaemonidae
Serotyping
Vibrio cholerae
Water Microbiology
Animalia
Bacteria (microorganisms)
Negibacteria
Vibrio
Vibrio cholerae
Vibrio mimicus
Issue Date: 2011
Abstract: Seven different monoclonal antibodies (MAbs) specific to only Vibrio cholerae were produced using a combination of five representative serotypes of V. cholerae for immunization. The first three MAbs (VC-93, VC-82 and VC-223) were specific to the V. cholerae serogroup O1 with different avidity for the serotypes O1 Inaba and O1 Ogawa. The fourth and the fifth MAbs were specific to V. cholerae O139 (VC-812) or O141 (VC-191) serogroups, respectively. The sixth MAb (VC-26) bound to all three serogroups of V. cholerae. The seventh MAb (VC-63) bound to all twenty five isolates of V. cholerae used in this study. None of the seven MAbs showed cross-reactivity with other Vibrio spp. or closely-related V. cholerae species, V. mimicus or other gram-negative bacteria. The eighth MAbs (VC-201) specific to almost all Vibrio spp. was also obtained. In dot blotting, these MAbs can be used to detect a diluted pure culture of V. cholerae in solution with a sensitivity range of from 10 5 to 10 7CFUml -1. However, the detection capability could be improved equivalent to that of PCR technique after preincubation of samples in alkaline peptone water (APW). Thus, these MAbs constitute convenient immunological tools that can be used for simple, rapid and simultaneous direct detection and differentiation of the individual serotypes of V. cholerae in complex samples, such as food and infected animals, without the requirement for bacterial isolation or biochemical characterization. © 2011 Elsevier B.V.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-80053573745&doi=10.1016%2fj.mimet.2011.07.022&partnerID=40&md5=2ad4a51752b5c44e7a75d4757c9573b1
http://ir.swu.ac.th/jspui/handle/123456789/14459
ISSN: 1677012
Appears in Collections:SCOPUS 1983-2021

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