Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/14429
Title: Detection of Mycobacterium tuberculosis by using loop-mediated isothermal amplification combined with a lateral flow dipstick biosensor
Authors: Kaewphinit T.
Santiwatanakul S.
Jaratsing P.
Chansiri K.
Arunrut N.
Kiatpathomchai W.
Keywords: Amplicons
Constant temperature
Developing world
DNA extraction
Ethidium bromide
Lateral Flow
Lateral flows
LFD
M. tuberculosis
Mycobacterium tuberculosis
Oligonucleotide probes
PCR assay
Test-lines
Biomedical engineering
Biosensors
Bromine compounds
Developing countries
Electric lamps
Electrophoresis
Genes
Isotherms
Oligonucleotides
Polymerase chain reaction
Tubes (components)
Issue Date: 2011
Abstract: Tuberculosis is a persistent problem in the developing world and the biggest cause of mortality. Loop-mediated isothermal amplification (LAMP) allows DNA to be amplified rapidly at a constant temperature. Here a LAMP method was combined with a chromatographic lateral-flow dipstick (LFD) to detect IS6110 gene of M. tuberculosis rapidly and specifically. The reaction was optimized at 65°C for 90 min and amplified DNA hybridized to an FITC-labeled oligonucleotide probe for 5 min was detected at LFD test line 5 min after application. Excluding for the step of DNA extraction, test results could be generated within 1 h 40 min. In addition to advantages of short assay time, confirmation of amplicon identity by hybridization and elimination of electrophoresis with carcinogenic ethidium bromide, the LAMP-LFD was more sensitive than an existing PCR assay for detection of M. tuberculosis. © 2011 IEEE.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84860449445&doi=10.1109%2fBMEiCon.2012.6172024&partnerID=40&md5=b1d727234d3f6228cfb979c74034ec88
http://ir.swu.ac.th/jspui/handle/123456789/14429
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