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Title: Detection of Mycobacterium tuberculosis by using loop-mediated isothermal amplification combined with a lateral flow dipstick biosensor
Authors: Kaewphinit T.
Santiwatanakul S.
Jaratsing P.
Chansiri K.
Arunrut N.
Kiatpathomchai W.
Keywords: Amplicons
Constant temperature
Developing world
DNA extraction
Ethidium bromide
Lateral Flow
Lateral flows
M. tuberculosis
Mycobacterium tuberculosis
Oligonucleotide probes
PCR assay
Biomedical engineering
Bromine compounds
Developing countries
Electric lamps
Polymerase chain reaction
Tubes (components)
Issue Date: 2011
Abstract: Tuberculosis is a persistent problem in the developing world and the biggest cause of mortality. Loop-mediated isothermal amplification (LAMP) allows DNA to be amplified rapidly at a constant temperature. Here a LAMP method was combined with a chromatographic lateral-flow dipstick (LFD) to detect IS6110 gene of M. tuberculosis rapidly and specifically. The reaction was optimized at 65°C for 90 min and amplified DNA hybridized to an FITC-labeled oligonucleotide probe for 5 min was detected at LFD test line 5 min after application. Excluding for the step of DNA extraction, test results could be generated within 1 h 40 min. In addition to advantages of short assay time, confirmation of amplicon identity by hybridization and elimination of electrophoresis with carcinogenic ethidium bromide, the LAMP-LFD was more sensitive than an existing PCR assay for detection of M. tuberculosis. © 2011 IEEE.
Appears in Collections:SCOPUS 1983-2021

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