Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/14384
Title: Development of a one-step immunochromatographic strip test using gold nanoparticles for the rapid detection of Salmonella typhi in human serum
Authors: Preechakasedkit P.
Pinwattana K.
Dungchai W.
Siangproh W.
Chaicumpa W.
Tongtawe P.
Chailapakul O.
Keywords: Analysis time
Control zones
Fiber membranes
Gold Nanoparticles
Human serum
Immunochromatographic strip test
Immunochromatographic strips
Limit of detection
Lower detection limit
Naked-eye
Nanoparticle conjugate
Nitrocellulose membranes
Rapid detection
Salmonella typhi
Sandwich immunoassay
Specific binding
Antibodies
Antigens
Body fluids
Cost accounting
Glass fibers
Gold
Immunology
Nanoparticles
Nitrocellulose
Salmonella
Testing
bacterium antibody
glass fiber
gold nanoparticle
immunoglobulin G antibody
pyroxylin
antibody labeling
antibody specificity
antigen binding
article
bacterium detection
blood analysis
coated particle
controlled study
diagnostic accuracy
enzyme immunoassay
human
immunoaffinity chromatography
immunoblotting
immunochromatographic strip test
intermethod comparison
limit of detection
nonhuman
process development
reaction time
Salmonella typhi
serum
Bacterial Load
Biosensing Techniques
Blood Chemical Analysis
Equipment Design
Equipment Failure Analysis
Gold
Humans
Immunochromatography
Nanoparticles
Nanotechnology
Reagent Strips
Reproducibility of Results
Salmonella typhi
Sensitivity and Specificity
Accounting
Antibodies
Binding
Cellulose Nitrate
Glass Fibers
Gold
Membranes
Salmonella typhi
Issue Date: 2012
Abstract: An immunochromatographic strip test using gold nanoparticles was developed for the rapid detection of Salmonella typhi (S. typhi) in human serum. The strip test based on the principle of sandwich immunoassay by the specific binding of antigens from S. typhi O901 and antibody of S. typhi O901 on a nitrocellulose membrane. Antibody-gold nanoparticle conjugate was used as the label and was coated onto a glass fiber membrane, which was used as a conjugate pad. To create a test and control zone, antibody of S. typhi O901 and an anti-IgG were dotted on the nitrocellulose membrane, respectively. Positive samples were displayed as red dots at the test and control zones of the nitrocellulose membrane, while negative samples resulted in a red dot only in the control zone. The limit of detection (LOD) was found to be 1.14×10 5cfumL -1, which could be visually detected by the naked eye within 15min. This strip test provided a lower detection limit and analysis time than a dot blot immunoassay (8.88×10 6cfumL -1 for LOD and 110min for reaction time). In addition, our immunochromatographic strip test was employed to detect S. typhi in human serum effectively, with high accuracy. This strip test offers great promise for a rapid, simple and low-cost analysis of S. typhi. © 2011 Elsevier B.V.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84455208100&doi=10.1016%2fj.bios.2011.10.031&partnerID=40&md5=84938ebbe31e1075304c2f4a1b4a6841
http://ir.swu.ac.th/jspui/handle/123456789/14384
ISSN: 9565663
Appears in Collections:Scopus 1983-2021

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