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Title: Characterization of mucus-associated proteins from abalone (Haliotis) - Candidates for chemical signaling
Authors: Kuanpradit C.
Stewart M.J.
York P.S.
Degnan B.M.
Sobhon P.
Hanna P.J.
Chavadej J.
Cummins S.F.
Keywords: glycine
mucus associated protein 1
mucus associated protein 2
mucus associated protein 3
secretory protein
unclassified drug
amino acid sequence
amino terminal sequence
animal experiment
animal tissue
chemical cue
controlled study
gene expression regulation
gene location
gene sequence
Haliotis asinina
juvenile animal
mass spectrometry
molecular recognition
molecular weight
mollusc larva
mucus associated protein gene
mucus secretion
nucleotide sequence
priority journal
protein aggregation
protein analysis
protein expression
protein function
protein secretion
protein stability
reproductive success
secretory cell
signal transduction
Signal Transduction
Species Specificity
Haliotis asinina
Issue Date: 2012
Abstract: Living in groups is a widespread phenomenon in the animal kingdom. For free-spawning aquatic animals, such as the abalone (Haliotis), being in the close proximity to potential mating partners enhances reproductive success. In this study, we investigated whether chemical cues could be present in abalone mucus that enable species-specific aggregation. A comparative MS analysis of mucus obtained from trailing or fixed stationary Haliotis asinina, and from seawater surrounding aggregations, indicated that water-soluble biomolecules are present and that these can stimulate sensory activity in conspecifics. Purified extracts of trail mucus contain at least three small proteins [termed H. asinina mucus-associated proteins (Has-MAPs)-1-3], which readily diffuse into the surrounding seawater and evoke a robust cephalic tentacle response in conspecifics. Mature Has-MAP-1 is approximately 9.9 kDa in size, and has a glycine-rich N-terminal region. Has-MAP-2 is approximately 6.2 kDa in size, and has similarities to schistosomin, a protein that is known to play a role in mollusc reproduction. The mature Has-MAP-3 is approximately 12.5 kDa in size, and could only be identified within trail mucus of animals outside of the reproductive season. All three Has-MAP genes are expressed at high levels within secretory cells of the juvenile abalone posterior pedal gland, consistent with a role in scent marking. We infer from these results that abalone mucus-associated proteins are candidate chemical cues that could provide informational cues to conspecifics living in close proximity and, given their apparent stability and hydrophilicity, animals further afield. © 2011 FEBS. No claim to original Australian government works.
ISSN: 1742464X
Appears in Collections:Scopus 1983-2021

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