Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/14315
Title: Evaluation of biomimetic scaffold of gelatin-hydroxyapatite crosslink as a novel scaffold for tissue engineering: Biocompatibility evaluation with human PDL fibroblasts, human mesenchymal stromal cells, and primary bone cells
Authors: Rungsiyanont S.
Dhanesuan N.
Swasdison S.
Kasugai S.
Keywords: Alkaline phosphatase activity
Apatite crystals
Biomimetic scaffolds
Bone cells
Cell types
Cellular response
Composite scaffolds
Crosslinked
Crosslinks
Fibroblast cells
gelatin-hydroxyapatite
HA powders
Hard tissues
Mesenchymal stromal cells
Morphological characteristic
Periodontal ligament
Pore configuration
Primary cells
Three dimensional cell culture
Biocompatibility
Biomimetics
Cell culture
Fibroblasts
Hydroxyapatite
Phosphatases
Phosphate minerals
Scanning electron microscopy
Tissue
Tissue engineering
Scaffolds (biology)
gelatin
hydroxyapatite
tissue scaffold
article
biomimetics
cell adhesion
cell culture
cell proliferation
chemistry
cytology
evaluation
fibroblast
human
mesenchymal stroma cell
osteoblast
periodontal ligament
scanning electron microscopy
tissue engineering
Biomimetics
Cell Adhesion
Cell Proliferation
Cells, Cultured
Durapatite
Fibroblasts
Gelatin
Humans
Mesenchymal Stromal Cells
Microscopy, Electron, Scanning
Osteoblasts
Periodontal Ligament
Tissue Engineering
Tissue Scaffolds
Issue Date: 2012
Abstract: Biomimetic gelatin (gel)-hydroxyapatite (HA) composites have been prepared for studying hard tissue engineering scaffolds. However, the biocompatibility test of this form of material using these three cell types, which are periodontal ligament (PDL) fibroblast cells, human mesenchymal stromal cells (HMSc) and primary cells from human hip bone (HBc) has never been evaluated. The objective of this article is to prepare and evaluate the biocompatibility of gel-HA crosslinked scaffold for tissue engineering. Two different scaffolds were prepared: preparation (1), 2.5% gel/2.5% HA; preparation (2), 2.5% gel/5% HA. Three cell types including PDL, HMSc, and HBc were used. Assessment of biocompatibility and osteoblastic cellular responses was evaluated using a three-dimensional cell culture method and scanning electron microscopy (SEM). From SEM, it was observed that scaffold (1) exhibits stable porous formation with well-blended and dispersed HA powder. All three cell types were able to proliferate in both scaffolds. The HMSc and HBc got attached to the scaffolds to a significantly higher degree and subsequently proliferated more than PDL. The alkaline phosphatase (ALP) activities of HMSc and HBc were stronger when cultured in scaffold (S1) than (S2). It was seen that the two scaffold preparations show good biocompatibility with all three cell types tested. The better cellular responses with scaffold (S1) than (S2) might be due to the different structural and morphological characteristics, that is, scaffold (S1) retained more small-sized apatite crystals and a better developed pore configuration than scaffold (S2). Based on these findings, the biomimetically synthesized composite scaffolds have the potential to be used in hard tissue regeneration and tissue engineering fields. © The Author(s) 2010 Reprints and permissions.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84863653015&doi=10.1177%2f0885328210391920&partnerID=40&md5=f2da7828a7030a0bae8023f75e79a194
http://ir.swu.ac.th/jspui/handle/123456789/14315
ISSN: 8853282
Appears in Collections:Scopus 1983-2021

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