Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/14261
Title: Restoration of auditory evoked responses by human ES-cell-derived otic progenitors
Authors: Chen W.
Jongkamonwiwat N.
Abbas L.
Eshtan S.J.
Johnson S.L.
Kuhn S.
Milo M.
Thurlow J.K.
Andrews P.W.
Marcotti W.
Moore H.D.
Rivolta M.N.
Keywords: cytology
hair
neurology
acoustic nerve fiber
article
cell differentiation
electrophysiology
embryonic stem cell
evoked auditory response
hair cell
human
human cell
in vitro study
priority journal
Animals
Auditory Threshold
Cell Differentiation
Cell Line
Cells, Cultured
Cochlear Nerve
Deafness
Embryonic Stem Cells
Evoked Potentials, Auditory
Fibroblast Growth Factor 10
Fibroblast Growth Factor 3
Gene Expression Profiling
Gene Expression Regulation, Developmental
Gerbillinae
Hair Cells, Auditory
Humans
Mice
Patch-Clamp Techniques
Stem Cell Transplantation
Stem Cells
Issue Date: 2012
Abstract: Deafness is a condition with a high prevalence worldwide, produced primarily by the loss of the sensory hair cells and their associated spiral ganglion neurons (SGNs). Of all the forms of deafness, auditory neuropathy is of particular concern. This condition, defined primarily by damage to the SGNs with relative preservation of the hair cells, is responsible for a substantial proportion of patients with hearing impairment. Although the loss of hair cells can be circumvented partially by a cochlear implant, no routine treatment is available for sensory neuron loss, as poor innervation limits the prospective performance of an implant. Using stem cells to recover the damaged sensory circuitry is a potential therapeutic strategy. Here we present a protocol to induce differentiation from human embryonic stem cells (hESCs) using signals involved in the initial specification of the otic placode. We obtained two types of otic progenitors able to differentiate in vitro into hair-cell-like cells and auditory neurons that display expected electrophysiological properties. Moreover, when transplanted into an auditory neuropathy model, otic neuroprogenitors engraft, differentiate and significantly improve auditory-evoked response thresholds. These results should stimulate further research into the development of a cell-based therapy for deafness. © 2012 Macmillan Publishers Limited. All rights reserved.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84867337075&doi=10.1038%2fnature11415&partnerID=40&md5=5ec7bb608d6f55987a386157fd83c5e8
http://ir.swu.ac.th/jspui/handle/123456789/14261
ISSN: 280836
Appears in Collections:SCOPUS 1983-2021

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