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|Title:||Quartz crystal microbalance DNA based biosensor for the detection of brugia malayi|
Quartz crystal microbalances
Self assembled monolayers
Polymerase chain reaction
|Abstract:||This Lymphatic filariasis is the major public health problem caused by Brugia spp. by the conventional methods as PCR cannot discriminate well, takes a few days or several hours. This study demonstrates a sensitive and specific quartz crystal microbalance (QCM) biosensor combined with polymerase chain reaction (PCR) for diagnosis of Brugia malayi. The procedure concludes: formation of self-assembled monolayer (SAM) on gold quartz crystals surface, attachment of the avidin to activated carboxyl groups by EDC/NHS, attachment of the biotin-modified probe to the avidin, and hybridization of probe to the target PCR. The PCR-QCM biosensor system was more specific than a PCR-gel electrophoresis assay in detecting the DNA of B. malayi and B. pahangi. © 2012 IFSA.|
|Appears in Collections:||SCOPUS 1983-2021|
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