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Title: Quartz crystal microbalance DNA based biosensor for the detection of brugia malayi
Authors: Kaewphinit T.
Santiwatanakul S.
Areekit S.
Chansiri K.
Keywords: B. malayi
Bio-sensor systems
Carboxyl groups
Conventional methods
DNA immobilization
DNA-based biosensors
Lymphatic filariasis
Quartz crystal
Quartz crystal microbalances
Self assembled monolayers
Polymerase chain reaction
Issue Date: 2012
Abstract: This Lymphatic filariasis is the major public health problem caused by Brugia spp. by the conventional methods as PCR cannot discriminate well, takes a few days or several hours. This study demonstrates a sensitive and specific quartz crystal microbalance (QCM) biosensor combined with polymerase chain reaction (PCR) for diagnosis of Brugia malayi. The procedure concludes: formation of self-assembled monolayer (SAM) on gold quartz crystals surface, attachment of the avidin to activated carboxyl groups by EDC/NHS, attachment of the biotin-modified probe to the avidin, and hybridization of probe to the target PCR. The PCR-QCM biosensor system was more specific than a PCR-gel electrophoresis assay in detecting the DNA of B. malayi and B. pahangi. © 2012 IFSA.
ISSN: 17265479
Appears in Collections:SCOPUS 1983-2021

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