Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/14188
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dc.contributor.authorJampasa S.
dc.contributor.authorWonsawat W.
dc.contributor.authorRodthongkum N.
dc.contributor.authorSiangproh W.
dc.contributor.authorYanatatsaneejit P.
dc.contributor.authorVilaivan T.
dc.contributor.authorChailapakul O.
dc.date.accessioned2021-04-05T03:33:31Z-
dc.date.available2021-04-05T03:33:31Z-
dc.date.issued2014
dc.identifier.issn9565663
dc.identifier.other2-s2.0-84888784888
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84888784888&doi=10.1016%2fj.bios.2013.11.023&partnerID=40&md5=6dcdd5e75ab68554d7813174f4baa489
dc.identifier.urihttp://ir.swu.ac.th/jspui/handle/123456789/14188-
dc.description.abstractAn electrochemical biosensor based on an immobilized anthraquinone-labeled pyrrolidinyl peptide nucleic acid (acpcPNA) probe was successfully developed for the selective detection of human papillomavirus (HPV) type 16 DNA. A 14-mer acpcPNA capture probe was designed to recognize a specific 14 nucleotide region of HPV type 16 L1 gene. The redox-active label anthraquinone (AQ) was covalently attached to the N-terminus of the acpcPNA probe through an amide bond. The probe was immobilized onto a chitosan-modified disposable screen-printed carbon electrode via a C-terminal lysine residue using glutaraldehyde as a cross-linking agent. Hybridization with the target DNA was studied by measuring the electrochemical signal response of the AQ label using square-wave voltammetric analysis. The calibration curve exhibited a linear range between 0.02 and 12.0. μM with a limit of detection and limit of quantitation of 4 and 14. nM, respectively. This DNA sensing platform was successfully applied to detect the HPV type 16 DNA from a PCR amplified (240. bp fragment of the L1 gene) sample derived from the HPV type 16 positive human cancer cell line (SiHa), and failed to detect the HPV-negative c33a cell line. The sensor probe exhibited very high selectivity for the complementary 14 base oligonucleotide over the non-complementary oligonucleotides with sequences derived from HPV types 18, 31 and 33. The proposed sensor provides an inexpensive tool for the early stage detection of HPV type 16, which is an important biomarker for cervical cancer. © 2013 Elsevier B.V.
dc.subjectAmino acids
dc.subjectAromatic compounds
dc.subjectCell culture
dc.subjectCrosslinking
dc.subjectDNA
dc.subjectDrug products
dc.subjectElectrodes
dc.subjectGenes
dc.subjectKetones
dc.subjectOligonucleotides
dc.subjectPeptides
dc.subjectProbes
dc.subjectSensors
dc.subjectAcpcPNA
dc.subjectAnthraquinone
dc.subjectELectrochemical detection
dc.subjectHuman papillomavirus
dc.subjectScreen printed electrodes
dc.subjectPolymerase chain reaction
dc.subjectvirus DNA
dc.subjectamino terminal sequence
dc.subjectarticle
dc.subjectbinding affinity
dc.subjectcarboxy terminal sequence
dc.subjectcontrolled study
dc.subjectDNA cross linking
dc.subjectDNA determination
dc.subjectelectrochemistry
dc.subjectfluorescence microscopy
dc.subjecthigh performance liquid chromatography
dc.subjectHuman papillomavirus type 16
dc.subjectnonhuman
dc.subjectnucleic acid probe
dc.subjectpiezoelectricity
dc.subjectpolymerase chain reaction
dc.subjectpotentiometry
dc.subjectprotein binding
dc.subjectprotein conformation
dc.subjectsensitivity and specificity
dc.subjectHuman papillomavirus
dc.subjectHuman papillomavirus type 16
dc.subjectacpcPNA
dc.subjectAnthraquinone
dc.subjectElectrochemical detection
dc.subjectHuman papillomavirus
dc.subjectScreen-printed electrode
dc.subjectAnthraquinones
dc.subjectBiosensing Techniques
dc.subjectCarbon
dc.subjectCell Line, Tumor
dc.subjectDNA, Viral
dc.subjectElectrochemical Techniques
dc.subjectElectrodes
dc.subjectEquipment Design
dc.subjectHuman papillomavirus 16
dc.subjectHumans
dc.subjectLimit of Detection
dc.subjectNucleic Acid Hybridization
dc.subjectNucleic Acid Probes
dc.subjectPapillomavirus Infections
dc.subjectPeptide Nucleic Acids
dc.titleElectrochemical detection of human papillomavirus DNA type 16 using a pyrrolidinyl peptide nucleic acid probe immobilized on screen-printed carbon electrodes
dc.typeArticle
dc.rights.holderScopus
dc.identifier.bibliograpycitationBiosensors and Bioelectronics. Vol 54, (2014), p.428-434
dc.identifier.doi10.1016/j.bios.2013.11.023
Appears in Collections:Scopus 1983-2021

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