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Title: Role of altholactone in inducing type II apoptosis signalling pathway and expression of cancer-related genes in cervical carcinoma HeLa cell line
Authors: Uthaisang-Tanechpongtamb W.
Sriyabhaya P.
Wilairat P.
Keywords: caspase 3
caspase 8
caspase 9
cytochrome c
protein bcl 2
protein Bid
protein p53
bcl 2 gene
cancer chemotherapy
carcinoma cell
cell structure
chromatin condensation
controlled study
drug determination
drug efficacy
drug mechanism
drug structure
enzyme activation
enzyme activity
enzyme inhibition
enzyme release
gene activity
gene expression profiling
gene function
Goniothalamus macrophyllus
human cell
intracellular signaling
p53 gene
protein analysis
protein cleavage
uterine cervix carcinoma
Issue Date: 2013
Abstract: Goniothalamus species (Annonaceae) is a shrub that grows in the rainforest of tropical Asia. Several compounds have been isolated and exhibit the potential use for cancer treatment. In this work, altholactone isolated from Goniothalamus macrophyllus was investigated for its cytotoxicity, apoptosis signalling and the expression of cancer-related genes in the cervical carcinoma HeLa cells. Cytotoxicity was evaluated by MTT assay. Apoptotic characteristics were evaluated by morphological studies. Caspase-3 activity was detected using a fluorogenic substrate. Cytochrome c release from mitochondria and protein Bid were determined byWestern blotting and cancer-related genes expression by RT-PCR. The results demonstrated that altholactone was cytotoxic to HeLa (IC50 = 9.6 μmg/mL), and apoptotic cell death was manifested by appearance of chromatin condensation and caspase-3 activation, which was inhibited by specific inhibitors of both caspase-8 and -9. Release into the cytosol of cytochrome and cleavage of Bid occurred. Altholactone also caused a decrease in bcl-2 and an increase in p53 expression. These unique properties of altholactone suggest a potential for cancer chemotherapy. © 2013 International Federation for Cell Biology.
ISSN: 10656995
Appears in Collections:Scopus 1983-2021

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